Recombinant Anti-PGAM1 antibody [EPR8042] (ab129191)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8042] to PGAM1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PGAM1 antibody [EPR8042]
See all PGAM1 primary antibodies -
Description
Rabbit monoclonal [EPR8042] to PGAM1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human PGAM1 (N terminal). The exact sequence is proprietary.
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Positive control
- Jurkat, Human fetal muscle, A431, and Raji cell lysates; permeabilized Raji cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.50 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8042 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
Target
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Function
Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. -
Tissue specificity
In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. -
Sequence similarities
Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily. - Information by UniProt
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Database links
- Entrez Gene: 5223 Human
- Entrez Gene: 18648 Mouse
- Entrez Gene: 24642 Rat
- Omim: 172250 Human
- SwissProt: P18669 Human
- SwissProt: Q9DBJ1 Mouse
- SwissProt: P25113 Rat
- Unigene: 592599 Human
see all -
Alternative names
- BPG dependent PGAM 1 antibody
- BPG dependent PGAM1 antibody
- BPG-dependent PGAM 1 antibody
see all
Images
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All lanes : Anti-PGAM1 antibody [EPR8042] (ab129191) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Human fetal muscle tissue lysate
Lane 3 : A431 cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 29 kDa -
ab129191 at 1/100 dilution staining PGAM1 in permeabilized Raji cells by intracellular flow cytometry or a rabbit IgG negative control (green).
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab129191 has been referenced in 4 publications.
- Min Z et al. Protein Lysine Acetylation in Ovarian Granulosa Cells Affects Metabolic Homeostasis and Clinical Presentations of Women With Polycystic Ovary Syndrome. Front Cell Dev Biol 8:567028 (2020). PubMed: 33043000
- Zhao Y & Zhang S PGAM1 knockdown is associated with busulfan-induced hypospermatogenesis and spermatogenic cell apoptosis. Mol Med Rep 19:2497-2502 (2019). PubMed: 30720109
- Zhang D et al. Phosphoglycerate Mutase 1 Predicts the Poor Prognosis of Oral Squamous Cell Carcinoma and is Associated with Cell Migration. J Cancer 8:1943-1951 (2017). PubMed: 28819393
- Oslund RC et al. Bisphosphoglycerate mutase controls serine pathway flux via 3-phosphoglycerate. Nat Chem Biol 13:1081-1087 (2017). PubMed: 28805803