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PGC1 alpha protein (His tag) (ab124541)

MSCatalog No. MS1046

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Overview

Product name

PGC1 alpha protein (His tag)
See all PGC1 alpha products (3) ...

Protein description

Recombinant fragment corresponding to amino acids 481-798 of Human PGC1 alpha with a C-terminal His tag; Predicted MW 37.8 kDa excluding tag (UniProt Q9UBK2).

Uniprot accession

Q9UBK2

Molecular weight

37.800kDa

Protein length

318 amino acids

Expression host

E. coli

Properties

Purity

> 80%

Form

Liquid

Storage instructions

Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

Storage buffer

pH: 7.40
Constituents: 78% PBS, 20% Glycerol, 1.75% Sodium chloride

Concentration

Concentration information loading...

Sequence notes

TGELRDSDFS NEQFSKLPMF INSGLAMDGL FDDSEDESDK LSYPWDGTQS YSLFNVSPSC SSFNSPCRDS VSPPKSLFSQ RPQRMRSRSR SFSRHRSCSR SPYSRSRSRS PGSRSSSRSC YYYESSHYRH RTHRNSPLYV RSRSRSPYSR RPRYDSYEEY QHERLKREEY RREYEKRESE RAKQRERQRQ KAIEERRVIY VGKIRPDTTR TELRDRFEVF GEIEECTVNL RDDGDSYGFI TYRYTCDAFA ALENGYTLRR SNETDFELYF CGRKQFFKSN YADLDSNSDD FDPASTKSKY DSLDFDSLLK EAQRSLRR

Research areas

Metabolism >> Types of disease >> Metabolic disorders
Metabolism >> Types of disease >> Heart disease
Metabolism >> Types of disease >> Cancer
Metabolism >> Types of disease >> Obesity
Metabolism >> Types of disease >> Diabetes
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Fatty acids
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Nucleotide metabolism >> Molecular processes >> Mitochondrial transcription
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial Biogenesis
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Cardiovascular >> Atherosclerosis >> Diabetes associated
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors

  • SDS-PAGE - PGC1 alpha protein (His tag) (ab124541)SDS-PAGE - PGC1 alpha protein (His tag) (ab124541) image (enlarge)

  • Western blot - PGC1 alpha protein (His tag) (ab124541)Western blot - PGC1 alpha protein (His tag) (ab124541) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab124541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    SDS-PAGE

     SDS-PAGE: Use at an ass...Read more →

    SDS-PAGE: Use at an assay dependent concentration.

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    ELISA

     ELISA: Use at an assay d...Read more →

    ELISA: Use at an assay dependent concentration.

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    WB

     WB: Use at an assay depe...Read more →

    WB: Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

Application notes

This peptide can be used with studies using ab54481,ab72230 and ab106814.

Protein info

Function

Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis.

Tissue specificity

Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.

Sequence similarities

Contains 1 RRM (RNA recognition motif) domain.

Post-translational
modifications

Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter (By similarity). Phosphorylated by CLK2.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ9UBK2 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

PGC1 alpha protein (His tag) images:

  SDS-PAGE - PGC1 alpha protein (His tag) (ab124541)

SDS-PAGE - PGC1 alpha protein (His tag) (ab124541)

Lane 1 : Marker
Lane 2 : PGC1 alpha protein (His tag) (ab124541) at 1 µg

Gel: 10-20% acrylamide separation gel

Predicted band size : 38 kDa
Observed band size : 38 kDa

Stained with Coomassie Blue.

  Western blot - PGC1 alpha protein (His tag) (ab124541)

Western blot - PGC1 alpha protein (His tag) (ab124541)

All lanes : Anti-PGC1 alpha + beta antibody (ab72230) at 1 µg/ml

Lane 1 : Marker
Lane 2 : PGC1 alpha protein (His tag) (ab124541) at 0.01 µg

Secondary
Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 38 kDa
Observed band size : 38 kDa


Exposure time : 5 minutes

References for PGC1 alpha protein (His tag) (ab124541)

ab124541 has not yet been referenced specifically in any publications.

Publishing research using ab124541? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"