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PGC1 alpha protein (Tagged-His Tag) (ab92266)

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Overview

  • Product namePGC1 alpha protein (Tagged-His Tag)See all PGC1 alpha proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 678-798 of Human PGC1 alpha with a N terminal His tag, 121aa, 24kDa, Uniprot ID = Q9UBK2.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:reconstitution with 128 µl aqua dest.
  • Storage instructionsStore at 4°C. Upon reconstitution store at -80ºC.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesVIYVGKIRPDTTRTELRDRFEVFGEIEECTVNLRDDGDSY GFITYRYTCDA FAALENGYTLRRSNETDFELYFCGRKQ FFKSNYADLDSNSDDFDPASTKSK YDSLDFDSLLKEAQ RSLRR
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92266 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        L PGC 1alphaLEM6Ligand effect modulator 6
        Peroxisome proliferative activated receptor gamma coactivator 1Peroxisome proliferative activated receptor gamma coactivator 1 alphaPeroxisome proliferative activated receptor, gamma, coactivator 1Peroxisome proliferative activated receptor, gamma, coactivator 1 alphaPeroxisome proliferator activated receptor gamma coactivator 1 alphaPeroxisome proliferator-activated receptor gamma coactivator 1-alphaPGC 1 (alpha)PGC 1 alphaPGC 1vPGC-1-alphaPGC1PGC1(alpha)PGC1APGC1vPPAR gamma coactivator 1PPAR gamma coactivator 1 alphaPPAR gamma coactivator 1 alpha 3 ligand effect modulator 6PPAR gamma coactivator variant formPPAR-gamma coactivator 1-alphaPPARGC 1 alphaPPARGC-1-alphaPPARGC1PPARGC1APRGC1_HUMAN
      see all
  • FunctionTranscriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism.
  • Tissue specificityHeart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.
  • Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications    Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter (By similarity). Phosphorylated by CLK2.
    Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q9UBK2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    PGC1 alpha protein (Tagged-His Tag) images

    • SDS-PAGE - PGC1 alpha protein (Tagged-His Tag) (ab92266)
      SDS-PAGE - PGC1 alpha protein (Tagged-His Tag) (ab92266)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 24 kDa (purple). The protein-specific band is blue.

    References for PGC1 alpha protein (Tagged-His Tag) (ab92266)

    ab92266 has not yet been referenced specifically in any publications.

    Publishing research using ab92266 ? Please let us know so that we can cite the reference in this datasheet

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