![Western blot - PGK1 antibody [22C5D8] (ab113687) image](#PGK1-Primary-antibodies-ab113687-2.jpg)
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Product name
Anti-PGK1 antibody [22C5D8]
See all PGK1 products (6) ...
Description
Mouse monoclonal [22C5D8] to PGK1
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Immunogen
Full length native protein (purified)
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Near homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
Clonality
Monoclonal
Clone number
22C5D8
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Carbohydrate metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
Cardiovascular >> Blood >> Other
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Western blot - PGK1 antibody [22C5D8] (ab113687)
![Western blot - PGK1 antibody [22C5D8] (ab113687) image](/ps/datasheet/images/113/ab113687/PGK1-Primary-antibodies-ab113687-2.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab113687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC/IF: Use a concentration of 5 - 10 µg/ml.
Target
Function
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Involvement in disease
Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations.
Sequence similarities
Belongs to the phosphoglycerate kinase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP00558
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell migration-inducing gene 10 protein antibody
- MGC117307 antibody
- MGC8947 antibody
see all
Database links
- SwissProt: P00560 Saccharomyces cerevisiae
Anti-PGK1 antibody [22C5D8] images:
Western blot - PGK1 antibody [22C5D8] (ab113687)
Anti-PGK1 antibody [22C5D8] (ab113687) + Yeast whole cell lysate at 15 µg
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 45 kDa
References for Anti-PGK1 antibody [22C5D8] (ab113687)
ab113687 has not yet been referenced specifically in any publications.
Publishing research using ab113687? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"