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Overview

  • Product namePGK1 protein (Tagged-His Tag)See all PGK1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 1-409 of Human PGK1 with N terminal His tag. MWt 47kDa; UniProt ID = P00558
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitution with 86 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notes(Amino acid sequence (Sequence determined by 5' Sequencing))
    MSLSNKLTLDKLDVKGKRVVMRVDFN VPMKNNQITNNQRIKAAVPSIKFCLDN GAKSVVLMSHL GRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEV EK ACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKA EPAKIEAFRASLSKLGD VYVNDAFGTAHRAHSSMVGVN LPQKAGGFLMKKELNYFAKALESPERPFLAIL GGAKVA DKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLF DEEGAKI VKDLMSKAEKNGVKITLPVDFVTADKFDENA KTGQATVASGI PAGWMGLDCGPESSKKYAEAVTRAKQI VWNGPVGVFEWEAFARGTKALMDEVV KATSRGCITIIG GGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLP
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91707 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Cell migration-inducing gene 10 proteinMGC117307MGC8947
        MIG10pgk1PGK1_HUMANPGKAPhosphoglycerate kinase 1Primer recognition protein 2PRP 2
      see all
  • FunctionIn addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
  • Involvement in diseaseDefects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations.
  • Sequence similaritiesBelongs to the phosphoglycerate kinase family.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession P00558 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    PGK1 protein (Tagged-His Tag) images

    • SDS-PAGE - PGK1 protein (Tagged-His Tag) (ab91707)
      SDS-PAGE - PGK1 protein (Tagged-His Tag) (ab91707)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for PGK1 protein (Tagged-His Tag) (ab91707)

    ab91707 has not yet been referenced specifically in any publications.

    Publishing research using ab91707 ? Please let us know so that we can cite the reference in this datasheet

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