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Overview

  • Product namePGLS protein
  • Protein descriptionRecombinant full length Human PGLS (amino acids 1-258) with an N terminal His tag (278 amino acids with the tag); predicted mwt: 29.7 kDa (NCBI Accession No: NP_036220; UniProt ID: O95336).
  • Uniprot accessionO95336
  • Molecular weight29.700kDa inclusive of tags
  • Protein length258 amino acids
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesab101059 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
  • Mass spectrometryMALDI-TOF
  • E.C. Number3.1.1.31
  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0
  • Concentration information loading...
  • SequenceMGSSHHHHHH SSGLVPRGSH MAAPAPGLIS VFSSSQELGA ALAQLVAQRA ACCLAGARAR FALGLSGGSL VSMLARELPA AVAPAGPASL ARWTLGFCDE RLVPFDHAES TYGLYRTHLL SRLPIPESQV ITINPELPVE EAAEDYAKKL RQAFQGDSIP VFDLLILGVG PDGHTCSLFP DHPLLQEREK IVAPISDSPK PPPQRVTLTL PVLNAARTVI FVATGEGKAA VLKRILEDQE ENPLPAALVQ PHTGKLCWFL DEAAARLLTV PFEKHSTL
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab101059 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA ELISA: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab135771.

    • Protein info

    • Alternative names
        6 phosphogluconolactonase6PGL
  • RelevancePGLS belongs to the glucosamine/galactosamine-6-phosphate isomerase family, 6-phosphogluconolactonase subfamily. It is implicated in the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.
  • Cellular localizationCytoplasmic

    • PGLS protein images

    • SDS-PAGE - PGLS protein (ab101059)
      SDS-PAGE - PGLS protein (ab101059)
      15% SDS-PAGE showing ab10105 (3 µg)

    References for PGLS protein (ab101059)

    ab101059 has not yet been referenced specifically in any publications.

    Publishing research using ab101059 ? Please let us know so that we can cite the reference in this datasheet

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