Anti-PGP9.5 antibody [31A3] (ab20559)

Overview

• Product nameAnti-PGP9.5 antibody [31A3]See all PGP9.5 primary antibodies ...
• Description
  Mouse monoclonal [31A3] to PGP9.5
• SpecificityAb20559 stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibers in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumors of the DNES.
• Tested applicationsIHC-P, ELISA, WB more details
• Species reactivity
  Reacts with: Rat, Human
  
• Immunogen

  Native PGP 9.5 protein from brain

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone number31A3
• IsotypeIgG1
• Research Areas
  • Tags & Cell Markers
  • Cell Type Markers
  • Neuroscience Markers
  • Neuronal

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Soma marker

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Parkinson's disease
  • Parkin / PARK

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Deubiquitination

Applications

Target

• FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
• Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
• Involvement in diseaseDefects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.
• Sequence similaritiesBelongs to the peptidase C12 family.
• Post-translational
  modificationsO-glycosylated.
• Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.

Target information above from: UniProt accession P09936 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7345 Human
  • Entrez Gene: 29545 Rat
  • Omim: 191342 Human
  • SwissProt: P09936 Human
  • SwissProt: Q00981 Rat
  • Unigene: 518731 Human
  • Unigene: 107213 Rat

• Alternative names
  Gracile axonal dystrophy antibodyNeuron cytoplasmic protein 9.5 antibodyOTTHUMP00000218137 antibody

  OTTHUMP00000218139 antibodyOTTHUMP00000218140 antibodyOTTHUMP00000218141 antibodyPark 5 antibodyPark5 antibodyParkinson Disease 5 antibodyPGP 9.5 antibodyPGP 9.5 antibodyPGP9.5 antibodyPGP9.5 antibodyPGP95 antibodyProtein gene product 9.5 antibodyUbiquitin C terminal esterase L1 antibodyUbiquitin C terminal hydrolase (neuron specific) antibodyUbiquitin C terminal hydrolase antibodyUbiquitin carboxyl terminal esterase L1 antibodyUbiquitin carboxyl terminal hydrolase isozyme L1 antibodyUbiquitin carboxyl-terminal hydrolase isozyme L1 antibodyUbiquitin thioesterase L1 antibodyUbiquitin thiolesterase antibodyUbiquitin thiolesterase L1 antibodyUCH L1 antibodyUCH L1 antibodyUCH-L1 antibodyUCHL1 antibodyUCHL1_HUMAN antibody

  see all

Anti-PGP9.5 antibody [31A3] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [31A3] (ab20559)Image from Wang W et al., Neuroscience Letters 495 (2011) 88–92, 16 May 2011. Fig 2.; doi:10.1016/j.neulet.2011.02.057 with permission from Elsevier.
  Immunohistochemical analysis of rat emybronic rectal tissue, staining PGP9.5 with ab20559.
  
  After antigen retrieval, the sections were treated and incubated with primary antibody (1/200), followed by an HRP-conjugated donkey anti-mouse IgG (1/200). Staining was detected using DAB.