Overview
- Product nameAnti-PGP9.5 antibody [EPR4117]See all PGP9.5 primary antibodies ...
- DescriptionRabbit monoclonal [EPR4117] to PGP9.5
- Tested applicationsWB, IP, IHC-P, ICC more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
A synthetic peptide corresponding to residues in Human PGP9.5.
- Positive controlFetal brain, Y79, U-87 MG, and SH SY5Y lysates; Human brain tissue.
- General notesProduced under U.S. Patent No. 5,675,063.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Dissociation constant (Kd) Kd = 7.20 x 10-10 M
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR4117
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab109261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa). |
| IP | IP: 1/10 - 1/100. |
| IHC-P | IHC-P: 1/50 - 1/100. Perform antigen retrieval before commencing with IHC staining protocol |
| ICC | ICC: 1/50 - 1/100. |
Target
- FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
- Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
- Involvement in diseaseDefects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.
- Sequence similaritiesBelongs to the peptidase C12 family.
- Post-translational
modificationsO-glycosylated. - Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
-
Database links
- Entrez Gene: 7345 Human
- Entrez Gene: 22223 Mouse
- Entrez Gene: 29545 Rat
- Omim: 191342 Human
- SwissProt: P09936 Human
- SwissProt: Q9R0P9 Mouse
- SwissProt: Q00981 Rat
- Unigene: 518731 Human
- Unigene: 29807 Mouse
- Unigene: 107213 Rat
see all
Target information above from: UniProt accession
P09936
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- Neuron cytoplasmic protein 9.5 antibodyOTTHUMP00000218137 antibodyOTTHUMP00000218139 antibody
- OTTHUMP00000218140 antibodyOTTHUMP00000218141 antibodyPark 5 antibodyPARK5 antibodyPGP 9.5 antibodyPGP9.5 antibodyPGP95 antibodyProtein gene product 9.5 antibodyUbiquitin C terminal esterase L1 antibodyUbiquitin C terminal hydrolase antibodyUbiquitin carboxyl terminal esterase L1 antibodyUbiquitin carboxyl terminal hydrolase isozyme L1 antibodyUbiquitin carboxyl-terminal hydrolase isozyme L1 antibodyUbiquitin thioesterase L1 antibodyUbiquitin thiolesterase antibodyUbiquitin thiolesterase L1 antibodyUCH-L1 antibodyUCHL1 antibodyUCHL1_HUMAN antibody
see all
Anti-PGP9.5 antibody [EPR4117] images
-
![Western blot - PGP9.5 antibody [EPR4117] (ab109261)](http://a.abcam.com/ps/datasheet/images/109/ab109261/PGP95-Primary-antibodies-ab109261-1.jpg)
Western blot - PGP9.5 antibody [EPR4117] (ab109261)All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Y79 lysate
Lane 3 : U-87 MG lysate
Lane 4 : SH SY5Y lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 24 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PGP9.5 antibody [EPR4117] (ab109261)](http://a.abcam.com/ps/datasheet/images/109/ab109261/PGP95-Primary-antibodies-ab109261-2.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PGP9.5 antibody [EPR4117] (ab109261)ab109261 at 1/50 dilution staining PGP9.5 in Human brain by Immunohistochemistry, Paraffin-embedded tissue. -
![Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)](http://a.abcam.com/ps/datasheet/images/109/ab109261/PGP95-Primary-antibodies-ab109261-12.jpg)
Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilution
Lane 1 : Whole tissue lysate prepared from adult mouse brain homogenate (CD-1 strain)
Lane 2 : Whole tissue lysate prepared from non transfected control cells (low endogenous expression of UCH-L1)
Lane 3 : Whole tissue lysate prepared from cells transfected with UCH-L1-Flag cDNA (positive control)
Lysates/proteins at 100 µg per lane.
Secondary
HRP conjugated goat anti-rabiit polyclonal at 1/10000 dilution
developed using the ECL technique
Predicted band size : 24 kDa
Exposure time : 2 minutesImage courtesy of Dr Simone Diestel by Abreview.
-
](http://a.abcam.com/ps/datasheet/images/109/ab109261/PGP95-Primary-antibodies-ab109261-14.jpg)
Other-Anti-PGP9.5 antibody [EPR4117](ab109261)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
References for Anti-PGP9.5 antibody [EPR4117] (ab109261)
ab109261 has not yet been referenced specifically in any publications.
