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Overview

  • Product nameAnti-PGP9.5 antibodySee all PGP9.5 primary antibodies ...
  • Description
    Chicken polyclonal to PGP9.5
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Recombinant full length human PGP9.5 purified from E. coli.

  • Positive controlRat hippocampal homogenate and Rat mixed neuron/glial cultures.

Properties

Applications

Our Abpromise guarantee covers the use of ab72910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/2500. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
ICC/IF ICC/IF: 1/500.

Target

  • FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • Involvement in diseaseDefects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.
  • Sequence similaritiesBelongs to the peptidase C12 family.
  • Post-translational
    modifications    O-glycosylated.
  • Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.

    • Target information above from: UniProt accession P09936 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      Gracile axonal dystrophy antibodyNeuron cytoplasmic protein 9.5 antibodyOTTHUMP00000218137 antibody
      OTTHUMP00000218139 antibodyOTTHUMP00000218140 antibodyOTTHUMP00000218141 antibodyPark 5 antibodyPARK5 antibodyParkinson Disease 5 antibodyPGP 9.5 antibodyPGP9.5 antibodyPGP95 antibodyProtein gene product 9.5 antibodyUbiquitin C terminal esterase L1 antibodyUbiquitin C terminal hydrolase (neuron specific) antibodyUbiquitin C terminal hydrolase antibodyUbiquitin carboxyl terminal esterase L1 antibodyUbiquitin carboxyl terminal hydrolase isozyme L1 antibodyUbiquitin carboxyl-terminal hydrolase isozyme L1 antibodyUbiquitin thioesterase L1 antibodyUbiquitin thiolesterase antibodyUbiquitin thiolesterase L1 antibodyUCH L1 antibodyUCH-L1 antibodyUCHL 1 antibodyUCHL1 antibodyUCHL1_HUMAN antibody
    see all

Anti-PGP9.5 antibody images

  • Western blot - PGP9.5 antibody (ab72910)
    Western blot - PGP9.5 antibody (ab72910)
    Anti-PGP9.5 antibody (ab72910) at 1/2500 dilution + rat hippocampal homogenate

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • Immunocytochemistry/ Immunofluorescence - PGP9.5 antibody (ab72910)
    Immunocytochemistry/ Immunofluorescence - PGP9.5 antibody (ab72910)
    ab72910 at 1/500 dilution staining PGP9.5 in rat mixed neuron/glial cultures by Immunofluorescence (green). Rabbit anti-GFAP antibody (red). Blue is a DNA stain.

References for Anti-PGP9.5 antibody (ab72910)

This product has been referenced in:
  • Fu YY  et al. 3-D imaging and illustration of mouse intestinal neurovascular complex. Am J Physiol Gastrointest Liver Physiol 304:G1-11 (2013). ICC/IF ; Mouse . Read more (PubMed: 23086917) »
  • Allen C  et al. Neutrophil cerebrovascular transmigration triggers rapid neurotoxicity through release of proteases associated with decondensed DNA. J Immunol 189:381-92 (2012). Read more (PubMed: 22661091) »

See all 2 Publications for this product

Publishing research using ab72910 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab72910 Submit a Question for ab72910

Displaying 1 - 2 of 2 results for Abreviews and Q&A

10
Votes: Highest First

I would like to test ab63103 for use in ICC.
Do you have a neuronal cell body marker that will work in ICC on rat cells and is not raised in rabbit or mouse?

Thank you for your phone call. For the neuronal cell body marker, I would recommend one of our chicken polyclonal to PGP9.5 antibodies: ab63497 or ab72910.

DISCOUNT CODE: ***
Expiration date: October 8th, 2012

I am very pleased t...

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Immunocytochemistry/ Immunofluorescence abreview for Anti-PGP9.5 antibody

 Poor
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human skin)
Specification human skin
Fixative Paraformaldehyde
Permeabilization Yes - triton 0.3 %
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Verified customer

Submitted Aug 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"