Anti-RBP-2 antibody (ab157096)
Key features and details
- Rabbit polyclonal to RBP-2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RBP-2 antibody -
Description
Rabbit polyclonal to RBP-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Guinea pig, Pig, Chimpanzee, Ferret, Non human primates, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human RBP-2 aa 344-394.
Database link: NP_115553.2 -
Positive control
- 293T, HeLa, Jurkat and NIH3T3 whole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 44 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 44 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA. -
Sequence similarities
Belongs to the PHAX family. -
Post-translational
modificationsPhosphorylated in the nucleus. Dephosphorylated in the cytoplasm (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nucleoplasm. Nucleus > Cajal body. Cytoplasm. Located in the nucleoplasm and Cajal bodies. Shuttles between the nucleus and the cytoplasm. Shuttles between the nucleoplasm and Cajal bodies. - Information by UniProt
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Database links
- Entrez Gene: 51808 Human
- Entrez Gene: 56698 Mouse
- Omim: 604924 Human
- SwissProt: Q9H814 Human
- SwissProt: Q9JJT9 Mouse
- Unigene: 555731 Human
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Alternative names
- FLJ13193 antibody
- PHAX antibody
- PHAX_HUMAN antibody
see all
Images
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All lanes : Anti-RBP-2 antibody (ab157096) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 10 seconds -
All lanes : Anti-RBP-2 antibody (ab157096) at 0.4 µg/ml
Lane 1 : NIH3T3 whole cell lysate at 50 µg
Lane 2 : NIH3T3 whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 3 minutes -
Immunoprecipitation. ab157096 at 0.1 µg/ml staining by Western blot RBP-2 in 293T whole cell lysate immunoprecipitated using ab157096 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
Predicted band size : 44 kDa.
Exposure time: 10 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab157096 has been referenced in 3 publications.
- Michalski D et al. Zika virus noncoding sfRNAs sequester multiple host-derived RNA-binding proteins and modulate mRNA decay and splicing during infection. J Biol Chem 294:16282-16296 (2019). PubMed: 31519749
- Sheng P et al. Dicer cleaves 5'-extended microRNA precursors originating from RNA polymerase II transcription start sites. Nucleic Acids Res 46:5737-5752 (2018). PubMed: 29746670
- Xie M et al. Mammalian 5'-capped microRNA precursors that generate a single microRNA. Cell 155:1568-80 (2013). PubMed: 24360278