Anti-PHF21B antibody [3D1] (ab119249)

Overview

• Product nameAnti-PHF21B antibody [3D1]See all PHF21B primary antibodies ...
• Description
  Mouse monoclonal [3D1] to PHF21B
• Tested applicationsWB, IHC-P, Flow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length Human PHF21B produced in HEK293T cells (NP_612424.1).

• Positive controlHuman Breast, Kidney, Endometrium, Liver, Ovary Adenocarcinoma, Pancreas carcinoma, Lung carcinoma and Thyroid Carcinoma tissues; HEK293T cell lysate transfected with pCMV6-ENTRY PHF21B cDNA; COS7 cells transiently transfected by pCMV6-ENTRY PHF21B; HEK293T cells transfected with pCMV6-ENTRY PHF21B overexpress plasmid.
• General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 48% PBS, 50% Glycerol, 1% BSA
• Concentration information loading...
• PurityProtein A purified
• Purification notesab119249 is purified from Mouse ascites fluid by affinity chromatography.
• Clonality Monoclonal
• Clone number3D1
• IsotypeIgG2b
• Research Areas
  • Cell Biology
  • Other Antibodies
  • Other Antibodies

Applications

Target

• Sequence similaritiesContains 1 PHD-type zinc finger.

Target information above from: UniProt accession Q96EK2 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 112885 Human
  • Entrez Gene: 112885 Human
  • SwissProt: Q96EK2 Human
  • SwissProt: Q96EK2 Human
  • Unigene: 254097 Human

• Alternative names
  A730032D07Rik antibodyAL049760.6 antibodyBHC80L antibody

  FLJ34161 antibodyOTTHUMP00000199355 antibodyOTTHUMP00000199357 antibodyPF21B_HUMAN antibodyPHD finger protein 21B antibodyPHD finger protein 4 antibodyPhf21b antibodyPHF4 antibodyRGD1308739 antibody

  see all

Anti-PHF21B antibody [3D1] images

• 

  Western blot - Anti-PHF21B antibody [3D1] (ab119249)
  All lanes : Anti-PHF21B antibody [3D1] (ab119249) at 1/2000 dilution
  
  Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
  Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PHF21B cDNA
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 57 kDa
  
• 

  Flow Cytometry - Anti-PHF21B antibody [3D1] (ab119249)
  ab119249 at 1/100, staining PHF21B in HEK293T cells transfected with either pCMV6-ENTRY PHF21B overexpress plasmid (Red) or empty vector control plasmid (Blue) by flow cytometry.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-PHF21B antibody [3D1] (ab119249)
  ab119249 at 1/100 dilution, staining PHF21B in COS7 cells transiently transfected with pCMV6-ENTRY PHF21B by Immunofluorescence.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [3D1] (ab119249)
  ab119249, at 1/150, staining PHF21B in paraffin-embedded Human Breast tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [3D1] (ab119249)
  ab119249, at 1/150, staining PHF21B in paraffin-embedded Human Endometrium tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [3D1] (ab119249)
  ab119249, at 1/150, staining PHF21B in paraffin-embedded Human Kidney tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [3D1] (ab119249)
  ab119249, at 1/150, staining PHF21B in paraffin-embedded Human Liver tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [3D1] (ab119249)
  ab119249, at 1/150, staining PHF21B in paraffin-embedded Human Lung Carcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [3D1] (ab119249)
  ab119249, at 1/150, staining PHF21B in paraffin-embedded Human Ovary Adenocarcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [3D1] (ab119249)
  ab119249, at 1/150, staining PHF21B in paraffin-embedded Human Pancreas Carcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [3D1] (ab119249)
  ab119249, at 1/150, staining PHF21B in paraffin-embedded Human Thyroid Carcinoma tissue by Immunohistochemistry.