Anti-PHF22 antibody (ab67885)

Overview

• Product nameAnti-PHF22 antibodySee all PHF22 primary antibodies ...
• Description
  Mouse polyclonal to PHF22
• SpecificityThis antibody is reactive against mammalian transfected lysate.
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  PHF22 (NP_065128.2, 1 a.a. ~ 462 a.a) full-length human protein.

• Positive controlPHF22 transfected 293T cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: Whole serum
• PurityWhole antiserum
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • Other

Applications

Target

• RelevancePHF22 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3' end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]). [OMIM]
• Cellular localizationNucleus
• Database links
  • Entrez Gene: 57117 Human
  • Omim: 611355 Human
  • SwissProt: Q96CB8 Human
  • Unigene: 480454 Human

• Alternative names
  INT12 antibodyIntegrator complex subunit 12 antibodyPHD finger protein 22 antibodySBBI22 antibody

Anti-PHF22 antibody images

• 

  Western blot - PHF22 antibody (ab67885)
  All lanes : Anti-PHF22 antibody (ab67885) at 1/500 dilution
  
  Lane 1 : PHF22 transfected 293T cell lysate
  Lane 2 : Non-transfected 293T cell lysate
  
  Lysates/proteins at 25 µg per lane.
  
  Secondary
  Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
  
  Predicted band size : 49 kDa
  Observed band size : 52 kDa (why is the actual band size different from the predicted?)