Anti-PHF8 antibody (ab84779)
Key features and details
- Rabbit polyclonal to PHF8
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-PHF8 antibody
See all PHF8 primary antibodies -
Description
Rabbit polyclonal to PHF8 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to a region between residue 650 and 700 of human PHF8 (CAI41576.1).
-
Positive control
- Human controls: Breast Carcinoma, Colon Carcinoma, Pancreatic Islet Cell Tumor, Prostate Carcinoma, Stomach Adenocarcinoma Mouse control: Teratoma
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab84779 was affinity purified using an epitope specific to PHF8 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500.
|
Notes |
---|
IHC-P
1/100 - 1/500. |
Target
-
Function
Histone lysine demethylase with selectivity for the di-and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3. -
Involvement in disease
Defects in PHF8 are the cause of mental retardation syndromic X-linked Siderius type (MRXSSD) [MIM:300263]. A disorder characterized by mild to borderline mental retardation with or without cleft lip/cleft palate. -
Sequence similarities
Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
Contains 1 JmjC domain.
Contains 1 PHD-type zinc finger. -
Domain
The PHD-type zinc finger mediates the binding to H3K4me3. Binding to H3K4me3 promotes its access to H3K9me2.
The linker region is a critical determinant of demethylase specificity. It enables the active site of JmjC to reach the target H3K9me2 when the PHD-type zinc finger binds to H3K4me3. -
Post-translational
modificationsPhosphorylation at Ser-69 and Ser-120 are required for dissociation from chromatin and accumulation of H4K20Me1 levels during prophase. -
Cellular localization
Nucleus. Nucleus > nucleolus. Recruited to H3K4me3 sites on chromatin during interphase. Dissociates from chromatin when cells enter mitosis. - Information by UniProt
-
Database links
- Entrez Gene: 23133 Human
- Entrez Gene: 320595 Mouse
- Entrez Gene: 74042 Mouse
- Omim: 300560 Human
- SwissProt: Q9UPP1 Human
- SwissProt: Q80TJ7 Mouse
- Unigene: 726110 Human
- Unigene: 17156 Mouse
see all -
Alternative names
- Histone lysine demethylase PHF8 antibody
- JHDM1F antibody
- Jumonji C domain containing histone demethylase 1F antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab84779 has been referenced in 1 publication.
- Gatchalian J et al. Chromatin condensation and recruitment of PHD finger proteins to histone H3K4me3 are mutually exclusive. Nucleic Acids Res 44:6102-12 (2016). ICC/IF ; Human . PubMed: 27016734