Overview

  • Product nameAnti-PHLDA3 antibodySee all PHLDA3 primary antibodies ...
  • Description
    Rabbit polyclonal to PHLDA3
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 38-87 (LQLFEAKGTG GRPKELSFAR IKAVECVEST GRHIYFTLVT EGGGEIDFRC) of Human PHLDA3 (NP_036528).

  • Positive control
    • 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab104766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Functionp53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor.
  • Tissue specificityWidely expressed with lowest expression in liver and spleen.
  • Sequence similaritiesBelongs to the PHLDA3 family.
    Contains 1 PH domain.
  • DomainThe PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.
  • Cellular localizationCytoplasm. Membrane.
  • Target information above from: UniProt accession Q9Y5J5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • PHLA3_HUMAN antibody
    • PHLDA 3 antibody
    • phlda3 antibody
    • Pleckstrin homology like domain family A member 2 antibody
    • Pleckstrin homology like domain family A member 3 antibody
    • Pleckstrin homology-like domain family A member 3 antibody
    • TDAG51/Ipl homolog 1 antibody
    • TDAG51/Ipl homologue 1 antibody
    • TIH 1 antibody
    • TIH1 antibody
    see all

Anti-PHLDA3 antibody images

  • Anti-PHLDA3 antibody (ab104766) at 1 µg/ml + 293T cell lysate at 10 µg

    Predicted band size : 14 kDa

References for Anti-PHLDA3 antibody (ab104766)

ab104766 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"