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Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)

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Overview

Product name

Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388]
See all PI 3 Kinase catalytic subunit gamma products (5) ...

Description

Rabbit monoclonal [Y388] to PI 3 Kinase catalytic subunit gamma

Specificity

This antibody is specific for PI 3 Kinase catalytic subunit gamma.

Tested applications

WB, Flow Cyt, IPmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

Synthetic peptide corresponding to residues in the N terminal of Human PI 3 Kinase catalytic subunit gamma.

Epitope

ab32089 reacts with an epitope located in the N terminal region of PI 3 Kinase catalytic subunit gamma

Positive control

Jurkat cell lysate.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

Y388

Isotype

IgG

Research areas

Immunology >> Innate Immunity >> TLR Signaling
Cancer >> Signal transduction >> G protein signaling >> GPCR
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Lipid Kinases

  • Western blot - PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)Western blot - PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Flow Cyt: 1/50.


IP: 1/50.
WB: 1/1000. Predicted molecular weight: 126 kDa.

Is unsuitable for ICC.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.

Tissue specificity

Pancreas, skeletal muscle, liver and heart.

Pathway

Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.

Sequence similarities

Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain.

Target information above from: UniProt accessionP48736 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 1 phosphatidylinositol 3 kinase antibody
  • 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma antibody
  • 5-bisphosphate 3-kinase catalytic subunit gamma isoform antibody
  • p110 gamma antibody
  • p110gamma antibody
  • p120 PI3K antibody
  • p120-PI3K antibody
  • Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit gamma isoform antibody
  • Phosphatidylinositol-4 antibody
  • Phosphoinositide 3 kinase catalytic gamma polypeptide antibody
  • PI3 kinase p110 subunit gamma antibody
  • PI3-kinase subunit gamma antibody
  • PI3CG antibody
  • PI3K antibody
  • PI3K-gamma antibody
  • PI3Kgamma antibody
  • PIK3 antibody
  • Pik3cg antibody
  • PK3CG_HUMAN antibody
  • PtdIns-3-kinase subunit gamma antibody
  • PtdIns-3-kinase subunit p110-gamma antibody
  • Serine/threonine protein kinase PIK3CG antibody
see all

Database links

Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] images:

  Western blot - PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)

Western blot - PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)

Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089) at 1/1000 dilution + Jurkat cell lysate.

Predicted band size : 126 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)

References for Anti-PI 3 Kinase catalytic subunit gamma antibody [Y388] (ab32089)

This product has been referenced in:

  • Conte E  et al. Inhibition of PI3K prevents the proliferation and differentiation of human lung fibroblasts into myofibroblasts: the role of class I P110 isoforms. PLoS One 6:e24663 (2011). WB; Human.Read more (PubMed: 21984893) »

See 1 publication for this product

Publishing research using ab32089? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"