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Overview

  • Product nameAnti-PI 3 Kinase p85 gamma antibodySee all PI 3 Kinase p85 gamma primary antibodies ...
  • Description
    Rabbit polyclonal to PI 3 Kinase p85 gamma
  • Tested applicationsELISA, WB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: LQEYHSQYQE KSKEYDRLYE EYTRTSQEIQ MKRTAIEAFN ETIKIFEEQC, corresponding to internal sequence amino acids 181-230 of Human PI 3 Kinase p85 gamma

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlTransfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab69870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution. The ELISA titre using a peptide based assay was 1/1562500.
    WB: Use at a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Alternative names
        DKFZp686P05226 antibodyp55 GAMMA antibodyp55PIK antibody
        Phosphatidylinositol 3 kinase regulatory gamma subunit antibodyPhosphoinositide 3 kinase regulatory subunit 3 (p55 gamma) antibodyPIK3R3 antibodyPtdIns 3 kinase p85 gamma antibody
      see all

    Anti-PI 3 Kinase p85 gamma antibody images

    • Western blot - PI 3 Kinase p85 gamma antibody (ab69870)
      Western blot - PI 3 Kinase p85 gamma antibody (ab69870)
      Anti-PI 3 Kinase p85 gamma antibody (ab69870) at 5 µg/ml + Transfected 293T cell lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size : 54 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 44 kDa (possible isoform).

    References for Anti-PI 3 Kinase p85 gamma antibody (ab69870)

    This product has been referenced in:
    • Zaman MS  et al. Inhibition of PTEN gene expression by oncogenic miR-23b-3p in renal cancer. PLoS One 7:e50203 (2012). WB ; Human . Read more (PubMed: 23189187) »

    See 1 Publication for this product

    Publishing research using ab69870 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"