Anti-PI 4 Kinase type 2 beta antibody (ab37812)

Overview

  • Product nameAnti-PI 4 Kinase type 2 beta antibodySee all PI 4 Kinase type 2 beta primary antibodies ...
  • Description
    Rabbit polyclonal to PI 4 Kinase type 2 beta
  • SpecificityThis antibody is specific for PI 4 Kinase type 2 beta.
  • Tested applicationsICC/IF, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from aa 1~17 of human PI 4 Kinase type 2 beta (N Terminal).

  • Positive control
    • HL-60 cell lysate and breast carcinoma tissue

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab37812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P 1/50 - 1/100.

Target

  • FunctionTogether with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the PI3/PI4-kinase family. Type II PI4K subfamily.
    Contains 1 PI3K/PI4K domain.
  • Cellular localizationCytoplasm. Membrane. Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac-GTP-dependent mechanism.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2610042N09Rik antibody
    • 4933409G22Rik antibody
    • FLJ11105 antibody
    • FLJ11105 antibody
    • P4K2B_HUMAN antibody
    • Phosphatidylinositol 4 kinase type 2 beta antibody
    • Phosphatidylinositol 4 kinase type II beta antibody
    • Phosphatidylinositol 4 kinase type II beta antibody
    • Phosphatidylinositol 4-kinase type 2-beta antibody
    • Phosphatidylinositol 4-kinase type II-beta antibody
    • PI4K 2 beta antibody
    • PI4K 2 beta antibody
    • PI4K 2b antibody
    • PI4K 2b antibody
    • PI4K II beta antibody
    • PI4K II beta antibody
    • PI4K IIb antibody
    • PI4K IIb antibody
    • Pi4k2b antibody
    • PI4KII-BETA antibody
    • PI4KIIb antibody
    • PIK42B antibody
    • Type II phosphatidylinositol 4 kinase beta isoform antibody
    • Type II phosphatidylinositol 4 kinase beta isoform antibody
    see all

Anti-PI 4 Kinase type 2 beta antibody images

  • Anti-PI 4 Kinase type 2 beta antibody (ab37812) at 1/100 dilution + HL-60 cell lysate

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • PI 4 Kinase type 2 beta expression detected on human breast cancer tissue using ab37812 at a 1/50 dilution (with a peroxidase-conjugated secondary antibody, followed by AEC staining).

    These data show the suitability of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • ICC/IF image of ab37812 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37812, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PI 4 Kinase type 2 beta antibody (ab37812)

ab37812 has not yet been referenced specifically in any publications.

Product Wall

Based on the very low homology (23%), ab37812 will very likely not detect mouse PI4K2B.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"