Overview

  • Product nameAnti-PI3 Kinase p110 beta antibody
    See all PI3 Kinase p110 beta primary antibodies
  • Description
    Mouse monoclonal to PI3 Kinase p110 beta
  • Tested applicationsIHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 147-257 of Human PI3 Kinase p110 beta

Properties

Applications

Our Abpromise guarantee covers the use of ab55593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 1µg for 106 cells.

Target

  • FunctionPhosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
  • Tissue specificityExpressed ubiquitously.
  • PathwayPhospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
  • Sequence similaritiesBelongs to the PI3/PI4-kinase family.
    Contains 1 PI3K/PI4K domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta antibody
    • 5-bisphosphate 3-kinase catalytic subunit beta isoform antibody
    • DKFZp779K1237 antibody
    • MGC133043 antibody
    • OTTHUMP00000216901 antibody
    • OTTHUMP00000216904 antibody
    • p110 BETA antibody
    • p110Beta antibody
    • Phosphatidylinositol 3 kinase catalytic beta polypeptide antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta antibody
    • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform antibody
    • Phosphatidylinositol-4 antibody
    • Phosphoinositide 3 kinase catalytic beta polypeptide antibody
    • PI3 kinase p110 subunit beta antibody
    • PI3-kinase subunit beta antibody
    • PI3K antibody
    • PI3K beta antibody
    • PI3K-beta antibody
    • PI3Kbeta antibody
    • PI3KCB antibody
    • PIK3C1 antibody
    • Pik3cb antibody
    • PK3CB_HUMAN antibody
    • PtdIns 3 kinase p110 antibody
    • PtdIns-3-kinase subunit beta antibody
    • PtdIns-3-kinase subunit p110-beta antibody
    see all

Anti-PI3 Kinase p110 beta antibody images

  • PI3 Kinase p110 beta antibody (ab55593) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymph node.
  • Western blot against tagged recombinant protein immunogen using ab55593 PI3 Kinase p110 beta antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa
  • Overlay histogram showing Jurkat cells stained with ab55593 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55593, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-PI3 Kinase p110 beta antibody (ab55593)

This product has been referenced in:
  • Höland K  et al. Targeting class IA PI3K isoforms selectively impairs cell growth, survival, and migration in glioblastoma. PLoS One 9:e94132 (2014). IHC . Read more (PubMed: 24718026) »
  • Wojtalla A  et al. Targeting the phosphoinositide 3-kinase p110-a isoform impairs cell proliferation, survival, and tumor growth in small cell lung cancer. Clin Cancer Res 19:96-105 (2013). IHC-P ; Human . Read more (PubMed: 23172887) »

See all 3 Publications for this product

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