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Anti-PIAS1 antibody (ab58403)

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Overview

Product name

Anti-PIAS1 antibody
See all PIAS1 products (5) ...

Description

Rabbit polyclonal to PIAS1

Tested applications

ELISA, WB, IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Synthetic peptide derived from the internal sequence of Human PIAS1.

Positive control

MDA-MB-435 cell extract and Human lung carcinoma tissue.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> Ubiquitin like Modifiers
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors

  • Western blot - PIAS1 antibody (ab58403)Western blot - PIAS1 antibody (ab58403) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - PIAS1 antibody (ab58403)Immunohistochemistry (Paraffin-embedded sections) - PIAS1 antibody (ab58403) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab58403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/10000.

    ELISA: 1/10000.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000. Dete...Read more →

    WB: 1/500 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

Target

Function

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling.

Tissue specificity

Expressed in numerous tissues with highest level in testis.

Pathway

Protein modification; protein sumoylation.

Sequence similarities

Belongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger.

Domain

The LXXLL motif is a transcriptional coregulator signature.
The SP-RING-type domain is required for promoting EKLF sumoylation.

Post-translational
modifications

Sumoylated.
Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene.

Cellular localization

Nucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.

Target information above from: UniProt accessionO75925 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DDXBP1 antibody
  • DEAD/H box binding protein 1 antibody
  • DEAD/H box-binding protein 1 antibody
  • E3 SUMO-protein ligase PIAS1 antibody
  • GBP antibody
  • Gu binding protein antibody
  • Gu-binding protein antibody
  • Pias1 antibody
  • PIAS1_HUMAN antibody
  • Protein inhibitor of activated STAT protein 1 antibody
  • RNA helicase II binding protein antibody
  • RNA helicase II-binding protein antibody
see all

Database links

Anti-PIAS1 antibody images:

  Western blot - PIAS1 antibody (ab58403)

Western blot - PIAS1 antibody (ab58403)

All lanes : Anti-PIAS1 antibody (ab58403) at 1/500 dilution

Lane 1 : MDA-MB-435 cell extract with no immunizing peptide
Lane 2 : MDA-MB-435 cell extract with immunizing peptide


Predicted band size : 72 kDa
Observed band size : 72 kDa

  Immunohistochemistry (Paraffin-embedded sections) - PIAS1 antibody (ab58403)

Immunohistochemistry (Paraffin-embedded sections) - PIAS1 antibody (ab58403)

ab58403, at 1/50 dilution, staining human PIAS1 in lung carcinoma tissue, by immunohistochemistry, in the presence or absence of the immunizing peptide. Paraffin-embedded tissue.

References for Anti-PIAS1 antibody (ab58403)

This product has been referenced in:

  • Trzcinska-Daneluti AM  et al. High-content functional screen to identify proteins that correct F508del-CFTR function. Mol Cell Proteomics 8:780-90 (2009). WB; Human.Read more (PubMed: 19088066) »

See 1 publication for this product

Publishing research using ab58403? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"