Anti-PIAS2 antibody [EPR2582(2)] (ab126601)

Overview

• Product nameAnti-PIAS2 antibody [EPR2582(2)]See all PIAS2 primary antibodies ...
• Description
  Rabbit monoclonal [EPR2582(2)] to PIAS2
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues in Human PIAS2.

• Positive controlHepG2 cell lysate; Human fetal kidney tissue lysate.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferpH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR2582(2)
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Ubiquitin & Ubiquitin Like Modifiers
  • Ubiquitin like Modifiers

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • STATs

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Sumo

Applications

Target

• FunctionFunctions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha.
• Tissue specificityMainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.
• PathwayProtein modification; protein sumoylation.
• Sequence similaritiesBelongs to the PIAS family.
  Contains 1 PINIT domain.
  Contains 1 SAP domain.
  Contains 1 SP-RING-type zinc finger.
• Developmental stageIsoform 3 expression in adult testis is 14.2-fold stronger than in embryonic testis.
• DomainThe LXXLL motif is a transcriptional coregulator signature.
• Post-translational
  modificationsSumoylated.
• Cellular localizationNucleus speckle. Nucleus > PML body. Colocalizes at least partially with promyelocytic leukemia nuclear bodies.

Target information above from: UniProt accession O75928 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9063 Human
  • Entrez Gene: 9063 Human
  • Entrez Gene: 9063 Human
  • Entrez Gene: 17344 Mouse
  • Entrez Gene: 83422 Rat
  • Omim: 603567 Human
  • SwissProt: O75928 Human
  • SwissProt: O75928 Human

  • SwissProt: O75928 Human
  • SwissProt: Q8C5D8 Mouse
  • SwissProt: Q6AZ28 Rat
  • Unigene: 658013 Human
  • Unigene: 6370 Mouse
  • Unigene: 25546 Rat

  see all

• Alternative names
  Androgen receptor interacting protein 3 antibodyAndrogen receptor-interacting protein 3 antibodyARIP3 antibody

  DAB2 interacting protein antibodyDAB2-interacting protein antibodyDIP antibodyE3 SUMO protein ligase PIAS2 antibodyE3 SUMO-protein ligase PIAS2 antibodyMIZ antibodyMiz1 antibodyMsx interacting zinc finger protein antibodyMsx-interacting zinc finger protein antibodyPIAS NY protein antibodyPIAS-NY protein antibodyPIAS2 antibodyPIAS2_HUMAN antibodyPIASX antibodyPIASX-ALPHA antibodyPIASX-BETA antibodyProtein inhibitor of activated STAT x antibodyProtein inhibitor of activated STAT, 2 antibodyProtein inhibitor of activated STAT2 antibodySIZ2 antibodyZinc finger, MIZ-type containing 4 antibodyZMIZ4 antibody

  see all

Anti-PIAS2 antibody [EPR2582(2)] images

• 

  Western blot - Anti-PIAS2 antibody [EPR2582(2)] (ab126601)
  All lanes : Anti-PIAS2 antibody [EPR2582(2)] (ab126601) at 1/1000 dilution
  
  Lane 1 : HepG2 cell lysate
  Lane 2 : Human fetal kidney tissue lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat anti-Rabbit HRP at 1/2000 dilution
  
  Predicted band size : 68 kDa