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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-PIAS3 antibody [PIA3]
See all PIAS3 products (7) ...
Description
Mouse monoclonal [PIA3] to PIAS3
Specificity
This antibody recognized PIAS3.
Tested applications
ELISA, ICC, WBmore details
Cross reactivity
Reacts with
Mouse, Chicken, Cow, Dog, Human
Immunogen
Synthetic peptide: GGALREGHGGPLPS, corresponding to amino acids 591-604 of Human PIAS3
GGALREGHGG PLPS
Positive control
HepG2 total cell extract.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified immunoglobulin.
Clonality
Monoclonal
Clone number
PIA3
Myeloma
NS1
Isotype
IgG1
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ICC
WB
Application notes
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 68 and additional band at 44 kDa may be present (predicted molecular weight: 67 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
Tissue specificity
Widely expressed.
Pathway
Protein modification; protein sumoylation.
Sequence similarities
Belongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger.
Domain
The LXXLL motif is a transcriptional coregulator signature.
Post-translational
modifications
Sumoylated.
Cellular localization
Nucleus speckle.
Target information above from: UniProt accessionQ9Y6X2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E3 SUMO protein ligase PIAS 3 antibody
- E3 SUMO protein ligase PIAS3 antibody
- E3 SUMO-protein ligase PIAS3 antibody
see all
Database links
- Entrez Gene: 10401 Human
- Entrez Gene: 229615 Mouse
- Omim: 605987 Human
- SwissProt: Q6IAR4 Human
- SwissProt: Q9Y6X2 Human
- SwissProt: O54714 Mouse
- Unigene: 435761 Human
- Unigene: 1635 Mouse
References for Anti-PIAS3 antibody [PIA3] (ab59547)
ab59547 has not yet been referenced specifically in any publications.
Publishing research using ab59547? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"