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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab22856? Please let us know so that we can cite the reference in this datasheet
ab22856 has been referenced in 2 publications.
- Borghouts C et al. The intracellular delivery of a recombinant peptide derived from the acidic domain of PIAS3 inhibits STAT3 transactivation and induces tumor cell death. Mol Cancer Res 8:539-53 (2010). WB; Mouse. PubMed: 20371673
- Lyst MJ et al. Regulation of MBD1-mediated transcriptional repression by SUMO and PIAS proteins. EMBO J 25:5317-28 (2006). WB; Human. PubMed: 17066076
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - PIAS3 antibody (ab22856)
Anti-PIAS3 antibody (ab22856) at 0.004 µg/ml + HepG2 cell lysate
Secondary
HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000
Performed under reducing conditions.
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PIAS3 antibody (ab22856)
ab22856, at a 1/125-250 dilution, staining human PIAS3 in human liver by Immunohistochemistry, Paraffin embedded tissue. Positive stained hepatocytes indicated by arrows. Magnification 400 x.
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