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Can you tell me the homology with mouse? |
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ANSWER: |
Thank you for your enquiry. The immunogen for this antibody is 100% homologous with mouse. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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I am looking for a PIAS3 antibody to use on mouse and bat tissues. Your anti-human PIAS3 antibody looks like the best tested for western blots, but I noticed it has only been tested on human tissue samples... Could I be sent a sample to test on our mouse and bat tissues for a western blot? |
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ANSWER: |
Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abpoints which can be redeemed on a number of rewards (a further 100 Abpoints will be offered for an image). Please contact us again if you have any additional questions. |
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What is the epitope recognized by this antibody? It states that the immunogen was a "synthetic peptide corresponding to a region of PIAS3" - what is that region? |
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ANSWER: |
Thank you for your enquiry and patience. The region of PIAS3 from which the peptide was chosen was: LSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYGPSVITSLDEQDALGHFFQY (from AA residue 499 to AA residue 553) If you have any additional questions, please contact us again.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-PIAS3 antibody (ab22856) at 0.004 µg/ml + HepG2 cell lysate
Secondary
HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000
Performed under reducing conditions.
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
ab22856, at a 1/125-250 dilution, staining human PIAS3 in human liver by Immunohistochemistry, Paraffin embedded tissue. Positive stained hepatocytes indicated by arrows. Magnification 400 x.
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