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Anti-PIAS3 antibody (ab22856)

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Overview

Product name

Anti-PIAS3 antibody
See all PIAS3 products (7) ...

Description

Rabbit polyclonal to PIAS3

Tested applications

WB, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

Rat, Cow, Dog

Immunogen

Synthetic peptide: HYGPSVITSL DEQDALGHFF QYRGTPSHFL GPLAPTLGSS HCSATPAPPP, corresponding to a sequence within C terminal amino acids 541-590 of Human PIAS3

HYGPSVITSL DEQDALGHFF QYRGTPSHFL GPLAPTLGSS HCSATPAPPP

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HepG2 cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> Ubiquitin like Modifiers
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors

  • Western blot - PIAS3 antibody (ab22856)Western blot - PIAS3 antibody (ab22856) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PIAS3 antibody (ab22856)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PIAS3 antibody (ab22856) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab22856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.

Tissue specificity

Widely expressed.

Pathway

Protein modification; protein sumoylation.

Sequence similarities

Belongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger.

Domain

The LXXLL motif is a transcriptional coregulator signature.

Post-translational
modifications

Sumoylated.

Cellular localization

Nucleus speckle.

Target information above from: UniProt accessionQ9Y6X2 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • E3 SUMO protein ligase PIAS 3 antibody
  • E3 SUMO protein ligase PIAS3 antibody
  • E3 SUMO-protein ligase PIAS3 antibody
  • FLJ14651 antibody
  • OTTHUMP00000015586 antibody
  • OTTHUMP00000015587 antibody
  • PIAS 3 antibody
  • Pias3 antibody
  • PIAS3 protein antibody
  • PIAS3_HUMAN antibody
  • Protein inhibitor of activated STAT 3 antibody
  • Protein inhibitor of activated STAT protein 3 antibody
  • Protein inhibitor of activated STAT3 antibody
  • Zinc finger MIZ type containing 5 antibody
  • ZMIZ 5 antibody
  • ZMIZ5 antibody
see all

Database links

see all

Anti-PIAS3 antibody images:

  Western blot - PIAS3 antibody (ab22856)

Western blot - PIAS3 antibody (ab22856)

Anti-PIAS3 antibody (ab22856) at 0.004 µg/ml + HepG2 cell lysate

Secondary
HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000

Performed under reducing conditions.

Observed band size : 68 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PIAS3 antibody (ab22856)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PIAS3 antibody (ab22856)

ab22856, at a 1/125-250 dilution, staining human PIAS3 in human liver by Immunohistochemistry, Paraffin embedded tissue. Positive stained hepatocytes indicated by arrows. Magnification 400 x.

References for Anti-PIAS3 antibody (ab22856)

This product has been referenced in:

  • Borghouts Cet al. The intracellular delivery of a recombinant peptide derived from the acidic domain of PIAS3 inhibits STAT3 transactivation and induces tumor cell death. Mol Cancer Res 8:539-53 (2010). WB; Mouse.Read more (PubMed: 20371673) »
  • Lyst MJet al. Regulation of MBD1-mediated transcriptional repression by SUMO and PIAS proteins. EMBO J 25:5317-28 (2006). WB; Human.Read more (PubMed: 17066076) »

See all 2 publications for this product

Publishing research using ab22856? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"