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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-PIAS3 antibody
See all PIAS3 products (7) ...
Description
Mouse monoclonal to PIAS3
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: PPTKKHCSVT SAAIPALPGS KGVLTSGHQP SSVLRSPAMG TLGGDFLSSL PLHEYPPAFP LGADIQGLDL FSFLQTESQH YGPSVITSLD EQDALGHF, corresponding to amino acids 453-551 of Human PIAS3
PPTKKHCSVT SAAIPALPGS KGVLTSGHQP SSVLRSPAMG TLGGDFLSSL PLHEYPPAFP LGADIQGLDL FSFLQTESQH YGPSVITSLD EQDALGHF
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
-
Western blot - PIAS3 antibody (ab56698)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
Tissue specificity
Widely expressed.
Pathway
Protein modification; protein sumoylation.
Sequence similarities
Belongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger.
Domain
The LXXLL motif is a transcriptional coregulator signature.
Post-translational
modifications
Sumoylated.
Cellular localization
Nucleus speckle.
Target information above from: UniProt accessionQ9Y6X2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E3 SUMO protein ligase PIAS 3 antibody
- E3 SUMO protein ligase PIAS3 antibody
- E3 SUMO-protein ligase PIAS3 antibody
see all
Database links
- Entrez Gene: 10401 Human
- Omim: 605987 Human
- SwissProt: Q6IAR4 Human
- SwissProt: Q9Y6X2 Human
- Unigene: 435761 Human
Anti-PIAS3 antibody images:
Western blot - PIAS3 antibody (ab56698)
Western blot against tagged recombinant protein immunogen using ab56698 PIAS3 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
References for Anti-PIAS3 antibody (ab56698)
ab56698 has not yet been referenced specifically in any publications.
Publishing research using ab56698? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"