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Immunohistochemistry (Paraffin-embedded sections) - PIAS3 antibody (ab58406)
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Western blot - PIAS3 antibody (ab58406)
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Product Name
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PIAS3 antibody
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See all PIAS3 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PIAS3
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Immunogen
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Synthetic peptide derived from Internal of human PIAS3.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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Detects endogenous levels of total PIAS3 protein
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human lung carcinoma tissue
293 cells treated with UV (5 mins)
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Cellular localization
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Mostly nuclear, in nuclear speckles.
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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PIAS3 is a member of the protein inhibitor of activated STAT (PIAS) family. It also activates TGF-beta/Smad transcriptional responses. Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PIAS3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PIAS3 antibody images, references, products related to ab58406 and other tools.
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PIAS3 antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - PIAS3 antibody (ab58406)
Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab58406 at 1/50-1/100 dilution. Samples were treated -/+ peptide.
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Western blot - PIAS3 antibody (ab58406)
All lanes : PIAS3 antibody (ab58406) at 1/500 dilution
Lane 1 : 293 cell extract treated with UV (5 min)
Lane 2 : 293 cell extract treated with UV (5 min) with peptide
Predicted band size : 67 kDa
Observed band size : 67 kDa
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Search PubMed (MEDLINE) for references to PIAS3
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PIAS3 antibody - more information
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Customer reviews (feedback) regarding PIAS3 antibody |
Customer FAQs regarding PIAS3 antibody |
Protocols for PIAS3 antibody |
Price and availability of products related to PIAS3 antibody |
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PIAS3 antibody - related products:
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PIAS3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PIAS3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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