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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-PIAS3 antibody
See all PIAS3 products (7) ...
Description
Rabbit polyclonal to PIAS3
Specificity
Detects endogenous levels of total PIAS3 protein
Tested applications
WB, IHC-P, ELISA, IPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide derived from Internal of human PIAS3.
Positive control
Human lung carcinoma tissue 293 cells treated with UV (5 mins)
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/10000ELISA: 1/10000
IP: Use at an assay dependent concentration.
Target
Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
Tissue specificity
Widely expressed.
Pathway
Protein modification; protein sumoylation.
Sequence similarities
Belongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger.
Domain
The LXXLL motif is a transcriptional coregulator signature.
Post-translational
modifications
Sumoylated.
Cellular localization
Nucleus speckle.
Target information above from: UniProt accessionQ9Y6X2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E3 SUMO protein ligase PIAS 3 antibody
- E3 SUMO protein ligase PIAS3 antibody
- E3 SUMO-protein ligase PIAS3 antibody
see all
Database links
- Entrez Gene: 10401 Human
- Entrez Gene: 229615 Mouse
- Entrez Gene: 83614 Rat
- Omim: 605987 Human
- SwissProt: Q6IAR4 Human
- SwissProt: Q9Y6X2 Human
- SwissProt: O54714 Mouse
- SwissProt: O70260 Rat
see all
Anti-PIAS3 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - PIAS3 antibody (ab58406)
Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab58406 at 1/50-1/100 dilution. Samples were treated -/+ peptide.
Western blot - PIAS3 antibody (ab58406)
All lanes : Anti-PIAS3 antibody (ab58406) at 1/500 dilution
Lane 1 : 293 cell extract treated with UV (5 min)
Lane 2 : 293 cell extract treated with UV (5 min) with peptide
Predicted band size : 67 kDa
Observed band size : 67 kDa
References for Anti-PIAS3 antibody (ab58406)
This product has been referenced in:
- Starsíchová Aet al. TGF-beta1 suppresses IL-6-induced STAT3 activation through regulation of Jak2 expression in prostate epithelial cells. Cell Signal 22:1734-44 (2010). WB; Human.Read more (PubMed: 20603212) »
See 1 publication for this product
Publishing research using ab58406? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"