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Products:Neuroscience >> Neurotransmission >> Secretory Vesicles >> Regulation
Overview
Product name
Anti-PICK1 antibody - N-terminal
See all PICK1 products (7) ...
Description
Rabbit polyclonal to PICK1 - N-terminal
Tested applications
ELISA, WB, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide from the N terminal region of human PICK1 conjugated to KLH
Positive control
Y79 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab71721 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Regulation
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Western blot - PICK1 antibody - N-terminal (ab71721)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).
Flow Cyt: Use at an assay dependent dilution.
Target
Function
Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel.
Tissue specificity
Ubiquitous.
Sequence similarities
Contains 1 AH domain.
Contains 1 PDZ (DHR) domain.
Post-translational
modifications
Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors.
Cellular localization
Cytoplasm > perinuclear region. Cell junction > synapse. Also present at excitatory synapses.
Target information above from: UniProt accessionQ9NRD5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- dJ1039K5 antibody
- MGC15204 antibody
- OTTHUMP00000028509 antibody
see all
Database links
- Entrez Gene: 9463 Human
- Entrez Gene: 18693 Mouse
- Omim: 605926 Human
- SwissProt: Q9NRD5 Human
- SwissProt: Q62083 Mouse
- Unigene: 180871 Human
- Unigene: 259464 Mouse
Anti-PICK1 antibody - N-terminal images:
Western blot - PICK1 antibody - N-terminal (ab71721)
Anti-PICK1 antibody - N-terminal (ab71721) at 1/100 dilution + Y79 cell lysate at 12.5 µg
Predicted band size : 47 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
References for Anti-PICK1 antibody - N-terminal (ab71721)
ab71721 has not yet been referenced specifically in any publications.
Publishing research using ab71721? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"