PICK1 antibody - N-terminal (ab71721)

Product Name 

PICK1 antibody - N-terminal

Product type 

Primary antibodies

Description 

Rabbit polyclonal to PICK1 - N-terminal

Immunogen 

Synthetic peptide from the N terminal region of human PICK1 conjugated to KLH

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

ELISA, Flow Cyt, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000.
Flow Cyt: Use at an assay dependent dilution.
WB: 1/100 - 1/500. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Y79 cell lysate.

Cellular localization 

Cytoplasmic; concentrated in the perinuclear region. Cell junction, synapse. Also present at excitatory synapses.

Research areas 

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Regulation

Relevance 

PSD-95/DLG/ZO-1 (PDZ) domain containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK 1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK 1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKC alpha), AMPA type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK 1 with PKC alpha is highly dependent on the activation of the kinase. It appears that PICK 1 directs the activated form of PKC alpha to membrane anchored GluR2. Once phosphorylated by PKC alpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK 1 dependent manner.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

ab71721 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for PICK1 antibody - N-terminal images, references, products related to ab71721 and other tools.

PICK1 antibody - N-terminal images:

Customer reviews (feedback) regarding PICK1 antibody - N-terminal

Customer FAQs regarding PICK1 antibody - N-terminal

Protocols for PICK1 antibody - N-terminal

Price and availability of products related to PICK1 antibody - N-terminal

PICK1 antibody - N-terminal - related products: