PICK1 antibody (ab15001)

Product Name 

PICK1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to PICK1

Immunogen 

6xhis tagged fusion protein: EKMELLDQKH VQDIVFQLQR FVSTMSKYYN DCYAVLQDAD VFPIEVDLAH TTLAYGPNQG SFTDGEEEDE EEEDGAAREV SKDACGATGP TDKGGSWCDS, corresponding to C terminal amino acids 317-416 of Mouse PICK1.
BLAST 'EKMELLDQKH VQDIVFQLQ R FVSTMSKY YN DCYAVLQ DAD VFPIEV DLAH TTLAY GPNQG SFTD GEEEDE EEE DGAAREV SK DACGATGP T ' with or

Reacts with 
(species key)

Ms, Rat

Specificity 

Recognizes PICK1.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: 1/500 - 1/1000. Predicted molecular weight: 50 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

RIPA lysates from rat brain microsomal preparation.

Cellular localization 

Cytoplasmic; concentrated in the perinuclear region. Cell junction, synapse. Also present at excitatory synapses.

Research areas 

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Regulation

Relevance 

PSD-95/DLG/ZO-1 (PDZ) domain containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK 1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK 1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKC alpha), AMPA type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK 1 with PKC alpha is highly dependent on the activation of the kinase. It appears that PICK 1 directs the activated form of PKC alpha to membrane anchored GluR2. Once phosphorylated by PKC alpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK 1 dependent manner.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 5mg/ml BSA, 0.15M Sodium Chloride, 50mM Glycine, 0.2mM Tris, 0.1mM EDTA. pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

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