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Products:Neuroscience >> Neurotransmission >> Secretory Vesicles >> Regulation
Overview
Product name
Anti-PICK1 antibody
See all PICK1 products (7) ...
Description
Rabbit polyclonal to PICK1
Specificity
Detects protein interacting with Protein Kinase C (PICK 1). By Western blot, this antibody detects an ~52 kDa protein representing PICK 1 from AtT20 cell extract. Immunocytochemical staining of CV1 cells transfected with PICK 1 results in a diffuse staining pattern when expressed alone and coclustered when coexpressed with mGluR7.
Tested applications
ICC, IP, WB, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: MFADLDYDIEEDKLGIPT, corresponding to amino acids 1-18 of Mouse PICK1.
MFADLDYDIE EDKLGIPT
Positive control
WB: AtT20 cell extract ICC: CV1 cells transfected with PICK 1
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA and 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Regulation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use a concentration of 1.7 µg/ml
IP: Use at an assay dependent dilution.
WB: Use a concentration of 2 µg/mlDetects a band of approximately 52 kDa.
ICC/IF: Use at an assay dependent concentration.
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Function
Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel.
Tissue specificity
Ubiquitous.
Sequence similarities
Contains 1 AH domain.
Contains 1 PDZ (DHR) domain.
Post-translational
modifications
Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors.
Cellular localization
Cytoplasm > perinuclear region. Cell junction > synapse. Also present at excitatory synapses.
Target information above from: UniProt accessionQ9NRD5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- dJ1039K5 antibody
- MGC15204 antibody
- OTTHUMP00000028509 antibody
see all
Database links
- Entrez Gene: 9463 Human
- Entrez Gene: 18693 Mouse
- Entrez Gene: 84591 Rat
- Omim: 605926 Human
- SwissProt: Q9NRD5 Human
- SwissProt: Q62083 Mouse
- SwissProt: Q9EP80 Rat
- Unigene: 180871 Human
see all
Anti-PICK1 antibody images:
Western blot - PICK1 antibody (ab3420)
Lane 1 : An myc antibody in PBST for 16 hours at 4°C
Lane 2 : Anti-PICK1 antibody (ab3420) at 1/500 dilution (in PBST for 16 hours at 4°C)
Lane 1 : Transfected CHO cells expressing human Pick1 tagged with myc
Lane 2 : Transfected CHO cells expressing human Pick1 tagged with myc
Lysates/proteins at 20 µg per lane.
Secondary
Lane 2 : An HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : ~52 kDa (why is the actual band size different from the predicted?)
Additional bands at : ~40 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
Blocking Step: 5% Milk for 1 hour at 20°C
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PICK1 antibody (ab3420)
IHC image of ab3420 staining in rat adult brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3420, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemistry/ Immunofluorescence - PICK1 antibody (ab3420)
ab3420 staining PICK1 in CHO cells stably transfected with Human PICK1 by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 1 hour at room temperature. The primary antibody was diluted 1/1000 in PBS, 0.5% Triton, 10% FCS and incubated with the sample for 2 hours at °C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/500.
This image is courtesy of an Anonymous Abreview.
References for Anti-PICK1 antibody (ab3420)
This product has been referenced in:
- Thorsen TSet al. Identification of a small-molecule inhibitor of the PICK1 PDZ domain that inhibits hippocampal LTP and LTD. Proc Natl Acad Sci U S A 107:413-8 (2010). IP; Human.Read more (PubMed: 20018661) »
See 1 publication for this product
Publishing research using ab3420? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"