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Western blot - PICK1 antibody (ab56586)
(enlarge)
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Product Name
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PICK1 antibody
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See all PICK1 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to PICK1
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Immunogen
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Recombinant fragment, corresponding to amino acids 266-376 of Human PICK1
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic; concentrated in the perinuclear region. Cell junction, synapse. Also present at excitatory synapses.
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Research areas
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Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Regulation
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Relevance
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PSD-95/DLG/ZO-1 (PDZ) domain containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK 1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK 1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKC alpha), AMPA type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK 1 with PKC alpha is highly dependent on the activation of the kinase. It appears that PICK 1 directs the activated form of PKC alpha to membrane anchored GluR2. Once phosphorylated by PKC alpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK 1 dependent manner.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PICK1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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PICK1 antibody images:
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 Western blot - PICK1 antibody (ab56586)
Western blot against tagged recombinant protein immunogen using ab56586 PICK1 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa
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Search PubMed (MEDLINE) for references to PICK1
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PICK1 antibody - more information
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Customer reviews (feedback) regarding PICK1 antibody |
Customer FAQs regarding PICK1 antibody |
Protocols for PICK1 antibody |
Price and availability of products related to PICK1 antibody |
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PICK1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PICK1 antibody |
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