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ab69767
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab69768 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - PIGA antibody (ab69768)
All lanes : Anti-PIGA antibody (ab69768) at 1 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 3 : HeLa Whole Cell Lysate - Hydroxyurea Treated (48hr, 2µM)
Lane 4 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 5 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 6 : HeLa Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 7 : HeLa Whole Cell Lysate - Hydroxyurea Treated (48hr, 1µM)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 54 kDa
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - PIGA antibody (ab69768)
ICC/IF image of ab69768 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69768, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, MCF-7 cells at 5µg/ml.
PIGA antibody for Western blot in Human (69768)
PIGA antibody for Immunocytochemistry/ Immunofluorescence in Mouse (69768)
Anti-PIGA antibody for Immunocytochemistry/ Immunofluorescence in Human (69768)
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