Overview
- Product nameAnti-PIGF antibody (Biotin)See all PiGF primary antibodies ...
- DescriptionRabbit polyclonal to PIGF (Biotin)
- ConjugationBiotin
- Specificityab84226 allows the detection of at least 0.2 – 0.4 ng/well of recombinant human PlGF in ELISA assays and 1.5 - 3.0 ng/lane in Western blot, under either reducing or non-reducing conditions.
- Tested applicationsWB, ELISA, Sandwich ELISA more details
- Species reactivityReacts with: Human
- Immunogen
Highly pure (>98%) recombinant human PlGF
- Positive controlRecombinant human PlGF.
Properties
- FormLyophilised:Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab84226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| ELISA | |
| Sandwich ELISA |
sELISA: Use at a concentration of 0.25 - 1 µg/ml (100 µl/well reaction).
WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 25 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionInvolved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI.
- PathwayGlycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
- Sequence similaritiesBelongs to the PIGF family.
- Cellular localizationEndoplasmic reticulum membrane.
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Database links
- Entrez Gene: 5281 Human
- Omim: 600153 Human
- SwissProt: Q07326 Human
- Unigene: 468415 Human
Target information above from: UniProt accession
Q07326
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- GPI11 homolog antibodyMGC32646 antibodyMGC33136 antibody
- PHOSPHATIDYLINOSITOL GLYCAN antibodyPhosphatidylinositol glycan biosynthesis class F protein antibodyPhosphatidylinositol-glycan biosynthesis class F protein antibodyPIG F antibodyPIG-F antibodyPIGF antibodyPIGF_HUMAN antibodyPIGF1 antibody
see all
References for Anti-PIGF antibody (Biotin) (ab84226)
ab84226 has not yet been referenced specifically in any publications.
