Anti-PIGK antibody (ab38443)

Overview

• Product nameAnti-PIGK antibodySee all PIGK primary antibodies ...
• Description
  Rabbit polyclonal to PIGK
• Tested applicationsELISA, WB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide (10-30 amino acids in length) conjugated to KLH, from the C terminal of last 100 amino acids of Human PIGK.

• Positive controlMouse liver tissue lysate, Human breast carcinoma tissue.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Purification notesThis antibody was purified through a protein G column, eluted out with both high and low pH buffers, neutralized immediately after elution then followed by dialysis against PBS.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • ER Proteins

Applications

Target

• RelevancePIGK is a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.
• Cellular localizationEndoplasmic reticulum, Endoplasmic reticulum membrane; type I single pass membrane protein
• Database links
  • Entrez Gene: 10026 Human
  • Entrez Gene: 10026 Human
  • Entrez Gene: 329777 Mouse
  • Omim: 605087 Human
  • SwissProt: Q92643 Human
  • SwissProt: Q92643 Human
  • SwissProt: Q9CXY9 Mouse

• Alternative names
  GPI anchor transamidase antibodyGPI transamidase antibodyGPI8 antibody

  Phosphatidylinositol glycan biosynthesis class K protein antibodyPhosphatidylinositol glycan class K antibodyPIG K antibody

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Anti-PIGK antibody images

• 

  Western blot - PIGK antibody (ab38443)
  Anti-PIGK antibody (ab38443) at 1/100 dilution + Mouse liver tissue lysate
  
  Predicted band size : 45 kDa
  Observed band size : 45 kDa
  Additional bands at : 25 kDa,37 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PIGK antibody (ab38443)
  ab38443, at 1/50 dilution, staining PIGK in Human breast carcinoma tissue by Immunohistochemistry, Formalin fixed and Paraffin embedded tissue. ab38443 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.