Anti-PIM1 (phospho Y309) antibody (ab74047)

Overview

• Product nameAnti-PIM1 (phospho Y309) antibodySee all PIM1 primary antibodies ...
• Description
  Rabbit polyclonal to PIM1 (phospho Y309)
• Specificityab74047 detects endogenous levels of human PIM1 only when phosphorylated at tyrosine 309. ab74047 detects endogenous levels of mouse PIM1 only when phosphorylated at tyrosine 302 and rat PIM1 only when phosphorylated at tyrosine 218.
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic phosphopeptide derived from human PIM1 around the phosphorylation site of tyrosine 309 (H-R-Y^P-H-G).

• Positive controlHUVEC cell extracts treated with PMA (125ng/ml, 30mins).

Properties

• FormLiquid
• Storage instructionsFrozen Stock (-20C). Shelf life 12 months.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab74047 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Hematopoietic Progenitors
  • Intracellular Molecules

  • Cancer
  • Signal transduction
  • Protein phosphorylation
  • Serine/threonine kinases
  • Other

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Signal transducers

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • Polycomb Silencing
  • Other

Applications

Target

• FunctionMay affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis.
• Tissue specificityExpressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
• Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsAutophosphorylated on both serine/threonine and tyrosine residues.
• Cellular localizationCytoplasm. Nucleus and Cell membrane.

Target information above from: UniProt accession P11309 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5292 Human
  • Entrez Gene: 18712 Mouse
  • Entrez Gene: 24649 Rat
  • Omim: 164960 Human
  • SwissProt: P11309 Human
  • SwissProt: P06803 Mouse
  • SwissProt: P26794 Rat
  • Unigene: 81170 Human

  • Unigene: 405293 Mouse
  • Unigene: 485038 Mouse
  • Unigene: 34888 Rat

  see all

• Alternative names
  Oncogene PIM 1 antibodyOncogene PIM1 antibodyPIM 1 antibody

  pim 1 kinase 44 kDa isoform antibodyPim 1 kinase antibodypim 1 oncogene (proviral integration site 1) antibodyPim 1 oncogene antibodyPIM antibodyPIM1 antibodypim1 kinase 44 kDa isoform antibodyPIM1_HUMAN antibodyPim2 antibodyPIM3 antibodyProto oncogene serine/threonine protein kinase Pim 1 antibodyProto-oncogene serine/threonine-protein kinase Pim-1 antibodyProviral integration site 1 antibodyProviral integration site 2 antibody

  see all

Anti-PIM1 (phospho Y309) antibody images

• 

  Western blot - PIM1 (phospho Y309) antibody (ab74047)
  All lanes : Anti-PIM1 (phospho Y309) antibody (ab74047) at 1/500 dilution
  
  Lane 1 : HUVEC cell extracts treated with PMA (125ng/ml, 30mins)
  Lane 2 : HUVEC cell extracts treated with PMA (125ng/ml, 30mins) with immunising peptide at 10 µg
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 35 kDa
  Observed band size : 35 kDa