Anti-PINK1 antibody [38CT18.7] (ab75487)

Overview

• Product nameAnti-PINK1 antibody [38CT18.7]See all PINK1 primary antibodies ...
• Description
  Mouse monoclonal [38CT18.7] to PINK1
• Tested applicationsICC/IF, WB, ELISA more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Recombinant full length PINK1 protein (Human).

• Positive control293 cell lysate transiently transfected with the PINK gene

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number38CT18.7
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitophagy fission and fusion

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Neurodegenerative disease

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Parkinson's disease
  • Other

Applications

Target

• FunctionProtects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitement to dysfunctional mitochondria to initiate their degradation.
• Tissue specificityHighly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.
• Involvement in diseaseDefects in PINK1 are the cause of Parkinson disease type 6 (PARK6) [MIM:605909]. A neurodegenerative disorder characterized by parkinsonian signs such as rigidity, resting tremor and bradykinesia. A subset of patients manifest additional symptoms including hyperreflexia, autonomic instability, dementia and psychiatric disturbances. Symptoms show diurnal fluctuation and can improve after sleep.
• Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsAutophosphorylated.
• Cellular localizationMitochondrion outer membrane. Cytoplasm > cytosol.

Target information above from: UniProt accession Q9BXM7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 65018 Human
  • Entrez Gene: 68943 Mouse
  • NCBI: 14165272 Human
  • Omim: 608309 Human
  • SwissProt: Q9BXM7 Human
  • SwissProt: Q99MQ3 Mouse
  • Unigene: 389171 Human
  • Unigene: 18539 Mouse

• Alternative names
  BRPK antibodyFLJ27236 antibodymitochondrial antibody

  PARK 6 antibodyPARK6 antibodyPhosphatase and Tensin Homolog antibodyPINK 1 antibodyPINK1 antibodyPINK1_HUMAN antibodyProtein kinase BRPK antibodyPTEN induced putative kinase 1 antibodyPTEN induced putative kinase protein 1 antibodyPTEN-induced putative kinase protein 1 antibodySerine/threonine kinase PINK1 mitochondrial antibodySerine/threonine protein kinase PINK1 mitochondrial antibodySerine/threonine-protein kinase PINK1 antibody

  see all

Anti-PINK1 antibody [38CT18.7] images

• 

  Western blot - PINK1 antibody [38CT18.7] (ab75487)
  All lanes : Anti-PINK1 antibody [38CT18.7] (ab75487) at 1/1000 dilution
  
  Lane 1 : Non-transfected 293 cell lysate
  Lane 2 : 293 cell lysate transiently transfected with the PINK1 gene
  
  Lysates/proteins at 2 µg per lane.
  
  
  Predicted band size : 63 kDa
  Observed band size : 63 kDa
  Additional bands at : ~50 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunocytochemistry/ Immunofluorescence - PINK1 antibody [38CT18.7] (ab75487)Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

  Immunocytochemistical detection of PINK1 antibody (ab75487) on Human Cell (PC12 cell line) Specification PC12 cell line Fixative: Paraformaldehyde.  Permeabilization: 0.3xTriton 100. Blocking step: 10% serum for 1 hour @ 4°C. Primary antibody incubated at 2 µg/ml for 24 hours @ 4°C. Secondary antibody: Alexa Fluor® 488 (1/1000). The submitted image shows PC12 cells stained with ab75487; DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  See Abreview