Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
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ab30903 |
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ab116177 |
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ab23707 has been referenced in 3 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-PINK1 antibody (ab23707) at 4 µg/ml + Murine liver 100,000 x g pellet at 30 µg
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Additional bands at : 33 kDa (possible cleavage fragment).
ICC/IF image of ab23707 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23707, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-PINK1 antibody (ab23707) at 1/1000 dilution + Whole cell lysate prepared from Jurkat cells at 100000 cells
Secondary
Donkey anti-rabbit IgG conjugated to HRP at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,35 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 seconds
Samples blocked with 5% milk for 1 hour at 25°C.
This image is courtesy of an anonymous Abreview
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