Overview

  • Product nameAnti-PINK1 antibody
    See all PINK1 primary antibodies
  • Description
    Rabbit polyclonal to PINK1
  • Tested applicationsWB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:

    LVRALLQREA SKRPSARVAA N

    , corresponding to amino acids 484-504 of Human PINK1.

  • Positive control
    • Human, mouse, rat liver.

Properties

Applications

Our Abpromise guarantee covers the use of ab23707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Detects a band of approximately 66 kDa.Can be blocked with Human PINK1 peptide (ab30903). Detects a band of approximately 66 kDa. Can be blocked with PINK1 peptide (484-504) (ab30903).
IHC-P Use a concentration of 4 µg/ml.
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • FunctionProtects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitement to dysfunctional mitochondria to initiate their degradation.
  • Tissue specificityHighly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.
  • Involvement in diseaseDefects in PINK1 are the cause of Parkinson disease type 6 (PARK6) [MIM:605909]. A neurodegenerative disorder characterized by parkinsonian signs such as rigidity, resting tremor and bradykinesia. A subset of patients manifest additional symptoms including hyperreflexia, autonomic instability, dementia and psychiatric disturbances. Symptoms show diurnal fluctuation and can improve after sleep.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationMitochondrion outer membrane. Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRPK antibody
    • BRPK antibody
    • FLJ27236 antibody
    • mitochondrial antibody
    • PARK 6 antibody
    • PARK6 antibody
    • Phosphatase and Tensin Homolog antibody
    • PINK 1 antibody
    • PINK1 antibody
    • PINK1_HUMAN antibody
    • Protein kinase BRPK antibody
    • PTEN induced putative kinase 1 antibody
    • PTEN induced putative kinase 1 antibody
    • PTEN induced putative kinase protein 1 antibody
    • PTEN-induced putative kinase protein 1 antibody
    • Serine/threonine kinase PINK1 mitochondrial antibody
    • Serine/threonine protein kinase PINK1 mitochondrial antibody
    • Serine/threonine-protein kinase PINK1 antibody
    see all

Anti-PINK1 antibody images

  • Anti-PINK1 antibody (ab23707) at 4 µg/ml + Murine liver 100,000 x g pellet at 30 µg

    Observed band size : 66 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa (possible cleavage fragment).
  • ICC/IF image of ab23707 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23707, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-PINK1 antibody (ab23707) at 1/1000 dilution + Whole cell lysate prepared from Jurkat cells at 100000 cells

    Secondary
    Donkey anti-rabbit IgG conjugated to HRP at 1/2000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 66 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-PINK1 antibody (ab23707)

This product has been referenced in:
  • Zou J  et al. Autophagy inhibitor LRPPRC suppresses mitophagy through interaction with mitophagy initiator Parkin. PLoS One 9:e94903 (2014). Human . Read more (PubMed: 24722279) »
  • DeVorkin L  et al. The Drosophila effector caspase Dcp-1 regulates mitochondrial dynamics and autophagic flux via SesB. J Cell Biol 205:477-492 (2014). Read more (PubMed: 24862573) »

See all 9 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Rat Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Dec 04 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Dec 04 2014



I have contacted the laboratories who have provided the IHC-P testing protocol which I hope will be helpful. Please note this would be a guideline only and may require individual optimization. I have copied it below.




I...

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Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step No blocking step used for 30 minute(s) · Concentration: 4% · Temperature: 16°C
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Submitted Apr 16 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Human Cell lysate - whole cell (SH-SY5Y human neuroblastoma)
Specification SH-SY5Y human neuroblastoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Sep 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (lung)
Loading amount 15 µg
Specification lung
Gel Running Conditions Reduced Denaturing (12% SDS page)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Apr 23 2013

Vielen Dank nochmal für Ihre Anfrage.



Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an, da wir bei über 100.000Produkten gar nicht die logistischen Möglichkeiten haben, neben unserer eigentliche...

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Vielen Dank für Ihre Anfrage.

Es tut mir leid zu hören, dass Sie Probleme mit diesen Antikörpern haben.

Unten finden Sie unserem Fragebogen. Durch das Ausfüllen des Fragebogens (gerne natürlich auch auf De...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skeletal Muscle)
Specification Skeletal Muscle
Fixative Methacarn
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 23°C
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Submitted Jan 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Skeletal Muscle)
Loading amount 20 µg
Specification Skeletal Muscle
Gel Running Conditions Reduced Denaturing (12% Bis-Tris gel)
Blocking step Li-Cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
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Submitted Dec 21 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"