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Western blot - PINK1 antibody (ab23707)
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Immunocytochemistry/ Immunofluorescence-PINK1 antibody(ab23707)
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Product Name
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PINK1 antibody
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See all PINK1 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PINK1
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Immunogen
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Synthetic peptide: LVRALLQREA SKRPSARVAA N, corresponding to amino acids 484-504 of Human PINK1.
BLAST 'LVRALLQREA SKRPSARVAA N' with  or 
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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CynMk
(Due to sequence homology)
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Specificity
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ab23707 reacts with PTEN induced putative kinase 1 (PINK1).
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
IHC-P: Use at a concentration of 4 µg/ml.
WB: Use at a concentration of 4 µg/ml. Detects a band of approximately 66 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human, mouse, rat liver.
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Cellular localization
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Mitochondrial
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Research areas
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Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
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Relevance
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PINK1 (PTEN-induced putative kinase 1) is a serine/threonine protein kinase that localises to mitochondria. It has been implicated in protecting cells from stress-induced mitochondrial dysfunction. Mutations in this gene are thought to be the cause of autosomal recessive early-onset Parkinson disease 6 (PARK6).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 0.1% BSA, TBS. pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.600 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PINK1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PINK1 antibody images, references, products related to ab23707 and other tools.
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PINK1 antibody images:
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 Immunocytochemistry/ Immunofluorescence-PINK1 antibody(ab23707)
ICC/IF image of ab23707 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23707, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to PINK1
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PINK1 antibody - more information
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Customer reviews (feedback) regarding PINK1 antibody |
Customer FAQs regarding PINK1 antibody |
Protocols for PINK1 antibody |
Price and availability of products related to PINK1 antibody |
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PINK1 antibody - related products:
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PINK1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PINK1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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