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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Overview
Product name
Anti-PINK1 antibody
See all PINK1 products (6) ...
Description
Rabbit polyclonal to PINK1
Specificity
ab23707 reacts with PTEN induced putative kinase 1 (PINK1).
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow, Cynomolgus Monkey
Immunogen
Synthetic peptide: LVRALLQREA SKRPSARVAA N, corresponding to amino acids 484-504 of Human PINK1.
LVRALLQREA SKRPSARVAA N
Positive control
Human, mouse, rat liver.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 0.1% BSA, Tris buffered saline, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/200Detects a band of approximately 66 kDa.Can be blocked with PINK1 peptide (484-504) (ab30903). (Use at a concentration of 4 µg/ml. Detects a band of approximately 66 kDa. Can be blocked with PINK1 peptide (484-504) (ab30903).)
IHC-P: Use a concentration of 4 µg/ml
ICC/IF: Use a concentration of 1 - 5 µg/ml.
Target
Function
Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitement to dysfunctional mitochondria to initiate their degradation.
Tissue specificity
Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.
Involvement in disease
Defects in PINK1 are the cause of Parkinson disease type 6 (PARK6) [MIM:605909]. A neurodegenerative disorder characterized by parkinsonian signs such as rigidity, resting tremor and bradykinesia. A subset of patients manifest additional symptoms including hyperreflexia, autonomic instability, dementia and psychiatric disturbances. Symptoms show diurnal fluctuation and can improve after sleep.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated.
Cellular localization
Mitochondrion outer membrane. Cytoplasm > cytosol.
Target information above from: UniProt accessionQ9BXM7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- BRPK antibody
- BRPK antibody
see all
Database links
- Entrez Gene: 65018 Human
- Entrez Gene: 68943 Mouse
- Entrez Gene: 298575 Rat
- NCBI: 14165272 Human
- Omim: 608309 Human
- SwissProt: Q9BXM7 Human
- SwissProt: Q99MQ3 Mouse
- Unigene: 389171 Human
see all
Anti-PINK1 antibody images:
Western blot - PINK1 antibody (ab23707)
Anti-PINK1 antibody (ab23707) at 4 µg/ml + Murine liver 100,000 x g pellet at 30 µg
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Additional bands at : 33 kDa (possible cleavage fragment).
Immunocytochemistry/ Immunofluorescence-PINK1 antibody(ab23707)
ICC/IF image of ab23707 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23707, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - PINK1 antibody (ab23707)
Anti-PINK1 antibody (ab23707) at 1/1000 dilution + Whole cell lysate prepared from Jurkat cells at 100000 cells
Secondary
Donkey anti-rabbit IgG conjugated to HRP at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,35 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 seconds
Samples blocked with 5% milk for 1 hour at 25°C.
This image is courtesy of an anonymous Abreview
References for Anti-PINK1 antibody (ab23707)
This product has been referenced in:
- Miñones-Moyano Eet al. MicroRNA profiling of Parkinson's disease brains identifies early downregulation of miR-34b/c which modulate mitochondrial function. Hum Mol Genet : (2011).Read more (PubMed: 21558425) »
- Yu Wet al. The PINK1/Parkin pathway regulates mitochondrial dynamics and function in mammalian hippocampal and dopaminergic neurons. Hum Mol Genet 20:3227-40 (2011). ICC/IF; Rat.Read more (PubMed: 21613270) »
See all 3 publications for this product
Publishing research using ab23707? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"