Overview
- Product nameAnti-PINK1 antibodySee all PINK1 primary antibodies ...
- DescriptionRabbit polyclonal to PINK1
- Specificityab23707 reacts with PTEN induced putative kinase 1 (PINK1).
- Tested applicationsWB, IHC-P, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Cynomolgus Monkey - Immunogen
Synthetic peptide: LVRALLQREA SKRPSARVAA N, corresponding to amino acids 484-504 of Human PINK1.
- Positive controlHuman, mouse, rat liver.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 0.1% BSA, Tris buffered saline, pH 7.4 -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab23707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/200 - 1/1000. Detects a band of approximately 66 kDa.Can be blocked with PINK1 peptide (484-504) (ab30903). Detects a band of approximately 66 kDa. Can be blocked with PINK1 peptide (484-504) (ab30903). |
| IHC-P | IHC-P: Use a concentration of 4 µg/ml. |
| ICC/IF | ICC/IF: Use a concentration of 1 - 5 µg/ml. |
Target
- FunctionProtects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitement to dysfunctional mitochondria to initiate their degradation.
- Tissue specificityHighly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.
- Involvement in diseaseDefects in PINK1 are the cause of Parkinson disease type 6 (PARK6) [MIM:605909]. A neurodegenerative disorder characterized by parkinsonian signs such as rigidity, resting tremor and bradykinesia. A subset of patients manifest additional symptoms including hyperreflexia, autonomic instability, dementia and psychiatric disturbances. Symptoms show diurnal fluctuation and can improve after sleep.
- Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain. - Post-translational
modificationsAutophosphorylated. - Cellular localizationMitochondrion outer membrane. Cytoplasm > cytosol.
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Database links
- Entrez Gene: 65018 Human
- Entrez Gene: 68943 Mouse
- Entrez Gene: 298575 Rat
- NCBI: 14165272 Human
- Omim: 608309 Human
- SwissProt: Q9BXM7 Human
- SwissProt: Q99MQ3 Mouse
- Unigene: 389171 Human
- Unigene: 18539 Mouse
see all
Target information above from: UniProt accession
Q9BXM7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- BRPK antibodyBRPK antibodyFLJ27236 antibody
- mitochondrial antibodyPARK 6 antibodyPARK6 antibodyPhosphatase and Tensin Homolog antibodyPINK 1 antibodyPINK1 antibodyPINK1_HUMAN antibodyProtein kinase BRPK antibodyPTEN induced putative kinase 1 antibodyPTEN induced putative kinase 1 antibodyPTEN induced putative kinase protein 1 antibodyPTEN-induced putative kinase protein 1 antibodySerine/threonine kinase PINK1 mitochondrial antibodySerine/threonine protein kinase PINK1 mitochondrial antibodySerine/threonine-protein kinase PINK1 antibody
see all
Anti-PINK1 antibody images
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Western blot - PINK1 antibody (ab23707)Anti-PINK1 antibody (ab23707) at 4 µg/ml + Murine liver 100,000 x g pellet at 30 µg
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Additional bands at : 33 kDa (possible cleavage fragment). -
Immunocytochemistry/ Immunofluorescence-PINK1 antibody(ab23707)ICC/IF image of ab23707 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23707, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - PINK1 antibody (ab23707)Anti-PINK1 antibody (ab23707) at 1/1000 dilution + Whole cell lysate prepared from Jurkat cells at 100000 cells
Secondary
Donkey anti-rabbit IgG conjugated to HRP at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,35 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 secondsThis image is courtesy of an anonymous Abreview
References for Anti-PINK1 antibody (ab23707)
This product has been referenced in:
- Jamart C et al. Modulation of autophagy and ubiquitin-proteasome pathways during ultra-endurance running. J Appl Physiol 112:1529-37 (2012). WB ; Human . Read more (PubMed: 22345427) »
- Miñones-Moyano E et al. MicroRNA profiling of Parkinson's disease brains identifies early downregulation of miR-34b/c which modulate mitochondrial function. Hum Mol Genet : (2011). Read more (PubMed: 21558425) »
