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Read our guarantee »Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Anti-PINK1 antibody
See all PINK1 products (6) ...
Rabbit polyclonal to PINK1
ab23707 reacts with PTEN induced putative kinase 1 (PINK1).
WB, IHC-P, ICC/IFmore details
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow, Cynomolgus Monkey
Synthetic peptide: LVRALLQREA SKRPSARVAA N, corresponding to amino acids 484-504 of Human PINK1.
LVRALLQREA SKRPSARVAA N
Human, mouse, rat liver.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 0.1% BSA, Tris buffered saline, pH 7.4
Concentration information loading...
Protein A purified
Polyclonal
IgG
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Our Abpromise guarantee covers the use of ab23707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/200Detects a band of approximately 66 kDa.Can be blocked with PINK1 peptide (484-504) (ab30903). (Use at a concentration of 4 µg/ml. Detects a band of approximately 66 kDa. Can be blocked with PINK1 peptide (484-504) (ab30903).)
IHC-P: Use a concentration of 4 µg/ml
ICC/IF: Use a concentration of 1 - 5 µg/ml.
Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitement to dysfunctional mitochondria to initiate their degradation.
Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.
Defects in PINK1 are the cause of Parkinson disease type 6 (PARK6) [MIM:605909]. A neurodegenerative disorder characterized by parkinsonian signs such as rigidity, resting tremor and bradykinesia. A subset of patients manifest additional symptoms including hyperreflexia, autonomic instability, dementia and psychiatric disturbances. Symptoms show diurnal fluctuation and can improve after sleep.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Autophosphorylated.
Mitochondrion outer membrane. Cytoplasm > cytosol.
Target information above from: UniProt accessionQ9BXM7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - PINK1 antibody (ab23707)

Anti-PINK1 antibody (ab23707) at 4 µg/ml + Murine liver 100,000 x g pellet at 30 µg
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Additional bands at : 33 kDa (possible cleavage fragment).
Immunocytochemistry/ Immunofluorescence-PINK1 antibody(ab23707)

ICC/IF image of ab23707 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23707, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - PINK1 antibody (ab23707)

Anti-PINK1 antibody (ab23707) at 1/1000 dilution + Whole cell lysate prepared from Jurkat cells at 100000 cells
Secondary
Donkey anti-rabbit IgG conjugated to HRP at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,35 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 seconds
Samples blocked with 5% milk for 1 hour at 25°C.
This image is courtesy of an anonymous Abreview
This product has been referenced in:
See all 3 publications for this product
Publishing research using ab23707? Please let us know so that we can cite the reference in this datasheet
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Concentration not available for this lot.
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Anti-PINK1 antibody (ab23707) at 4 µg/ml + Murine liver 100,000 x g pellet at 30 µg
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Additional bands at : 33 kDa (possible cleavage fragment).

ICC/IF image of ab23707 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23707, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PINK1 antibody (ab23707) at 1/1000 dilution + Whole cell lysate prepared from Jurkat cells at 100000 cells
Secondary
Donkey anti-rabbit IgG conjugated to HRP at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa,35 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 seconds
Samples blocked with 5% milk for 1 hour at 25°C.
This image is courtesy of an anonymous Abreview
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