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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Overview
Product name
PINK1 peptide (484-504)
See all PINK1 products (2) ...
Protein description
Synthetic peptide: LVRALLQREA SKRPSARVAA N, corresponding to amino acids 484-504 of Human PINK1 This peptide was used as an immunogen for ab23707 - PINK1 antibody.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.1% BSA, 200ul Tris buffered saline. pH 7.4
Concentration
Concentration information loading...
Sequence
LVRALLQREA SKRPSARVAA N
Research areas
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking
Application notes
BL: Use at an assay dependent dilution.
To block antibody/protein complex formation, the following procedure is recommended:
1) Mix the PINK1 polyclonal antibody (ab23707) and blocking peptide together in a 1:1 (v/v) ratio in a microfuge tube. For example, mix 40ul of antibody and 40ul of peptide*.
2) Incubate for 1 hour at room temperature with occasional mixing.
3) Dilute the reagents to the final working antibody concentration and apply to the slide or membrane as usual.
* This is a recommended mixture. The minimum amount of peptide needed for complete blocking has not been precisely determined and may vary depending on the sample being analyzed. The amount of peptide required may need to be increased if sufficient blocking does not occur.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitement to dysfunctional mitochondria to initiate their degradation.
Tissue specificity
Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.
Involvement in disease
Defects in PINK1 are the cause of Parkinson disease type 6 (PARK6) [MIM:605909]. A neurodegenerative disorder characterized by parkinsonian signs such as rigidity, resting tremor and bradykinesia. A subset of patients manifest additional symptoms including hyperreflexia, autonomic instability, dementia and psychiatric disturbances. Symptoms show diurnal fluctuation and can improve after sleep.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated.
Cellular localization
Mitochondrion outer membrane. Cytoplasm > cytosol.
Target information above from: UniProt accessionQ9BXM7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for PINK1 peptide (484-504) (ab30903)
ab30903 has not yet been referenced specifically in any publications.
Publishing research using ab30903? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"