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Product name
Anti-PIP5K1 beta antibody
See all PIP5K1 beta products (2) ...
Description
Rabbit polyclonal to PIP5K1 beta
Tested applications
IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 1-18 of Human PIP5K1 B.
Positive control
Human placenta cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Primary antibody notes
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Lipid Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/1000.ELISA: 1/1000.
WB: 1/100 - 1/500. Detects a band of approximately 61 kDa (predicted molecular weight: 64 kDa).
Target
Function
Mediates RAC1-dependent reorganization of actin filaments (By similarity). Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate.
Tissue specificity
Detected in heart, pancreas, brain, kidney, skeletal muscle and lung.
Sequence similarities
Contains 1 PIPK domain.
Cellular localization
Endomembrane system. Associated with membranes.
Target information above from: UniProt accessionO14986
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MSS 4 antibody
- Phosphatidylinositol 4 phosphate 5 kinase type 1 beta antibody
- Phosphatidylinositol-4-phosphate 5-kinase type I beta antibody
see all
Database links
- Entrez Gene: 8395 Human
- Omim: 602745 Human
- SwissProt: O14986 Human
- Unigene: 534371 Human
Anti-PIP5K1 beta antibody images:
Western blot - PIP5K1 beta antibody (ab5468)
Predicted band size : 64 kDa
ab5468 at a 1/100 dilution staining approximately 61kDa band of PIP5K1 B in human placenta cell lysate by Western blot (ECL).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PIP5K1 beta antibody (ab5468)
ab5468 staining PIP5K1 beta in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
References for Anti-PIP5K1 beta antibody (ab5468)
ab5468 has not yet been referenced specifically in any publications.
Publishing research using ab5468? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"