Anti-PIP5KI gamma antibody - N-terminal (ab71820)

Overview

• Product nameAnti-PIP5KI gamma antibody - N-terminalSee all PIP5KI gamma primary antibodies ...
• Description
  Rabbit polyclonal to PIP5KI gamma - N-terminal
• Tested applicationsELISA, IHC-P, WB more details
• Species reactivity
  Reacts with: Human
  
• Positive control293 cell lysate and human hepatocarcinoma tissue.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Purification notesab71820 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Signaling Pathway
  • Lipid Signaling
  • Lipid Kinases

Applications

Target

• FunctionPlays a role in membrane ruffling and assembly of clathrin-coated pits at the synapse. Mediates RAC1-dependent reorganization of actin filaments (By similarity). Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate.
• Involvement in diseaseDefects in PIP5K1C are the cause of lethal congenital contracture syndrome type 3 (LCCS3) [MIM:611369]; also known as multiple contractural syndrome Israeli Bedouin type B. LCCS is an autosomal recessive disorder characterized by early fetal hydrops and akinesia, the Pena-Shokeir phenotype, specific neuropathology with degeneration of anterior horn neurons and extreme skeletal muscle atrophy. LCCS3 patients present at birth with severe multiple joint contractures with severe muscle wasting and atrophy, mainly in the legs. LCCS3 can be distinguished from the original LCCS by the absence of hydrops, fractures, and multiple pterygia.
• Sequence similaritiesContains 1 PIPK domain.
• Cellular localizationCell membrane. Cytoplasmic, associated with the plasma membrane. Detected in focal adhesion plaques, membrane ruffles and plasma membrane invaginations.

Target information above from: UniProt accession O60331 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 23396 Human
  • Entrez Gene: 23396 Human
  • Entrez Gene: 23396 Human
  • Omim: 606102 Human
  • SwissProt: O60331 Human
  • SwissProt: O60331 Human
  • SwissProt: O60331 Human
  • Unigene: 282177 Human

• Alternative names
  diphosphoinositide kinase antibodyLCCS3 antibodyPhosphatidylinositol 4 phosphate 5 kinase type 1 gamma antibody

  Phosphatidylinositol-4-phosphate 5-kinase type I gamma antibodyPhosphatidylinositol-4-phosphate 5-kinase type-1 gamma antibodyPI51C antibodyPI51C_HUMAN antibodyPIP5K GAMMA antibodyPIP5K1 gamma antibodyPIP5K1-gamma antibodyPip5k1c antibodyPIP5K1C protein antibodyPIP5K1G antibodyPIP5KIgamma antibodyPtdIns(4)P 5 kinase gamma antibodyPtdIns(4)P-5-kinase 1 gamma antibodytype I PIP kinase antibody

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Anti-PIP5KI gamma antibody - N-terminal images

• 

  Western blot - PIP5KI gamma antibody - N-terminal (ab71820)
  Anti-PIP5KI gamma antibody - N-terminal (ab71820) at 1/100 dilution + 293 cell lysate at 12.5 µg
  
  Predicted band size : 74 kDa
  Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PIP5KI gamma antibody - N-terminal (ab71820)
  ab71820 at 1/50 dilution, staining PIP5KI gamma in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.