Overview
- Product nameAnti-PIST antibodySee all PIST primary antibodies ...
- DescriptionRabbit polyclonal to PIST
- Specificityab37036 recognises PIST.
- Tested applicationsWB, IHC-P, IHC-Fr, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide of 16 amino acid from near the carboxy terminus of PIST (Human)
- Positive controlRat colon cell lysates
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab37036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| IHC-P | |
| IHC-Fr | |
| ICC/IF |
IHC-P: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 51 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionPlays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.
- Tissue specificityUbiquitously expressed.
- Involvement in diseaseNote=A chromosomal aberration involving GOPC is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.
- Sequence similaritiesContains 1 PDZ (DHR) domain.
- DomainThe PDZ domain mediates interactions with FZD5, FZD8, ACCN3, GRID2, CLCN3 (By similarity). Mediates also interaction with CFTR and ADRB1.
The coiled-coil region probably mediates association to membranes, targeting to the Golgi, and interactions with GOLGA3, and STX6. May also mediate interaction with RHOQ. - Cellular localizationCytoplasm. Golgi apparatus membrane. Golgi apparatus > trans-Golgi network membrane. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Enriched in synaptosomal and postsynaptic densities (PSD) fractions (By similarity). Expressed in cell bodies and dendrites of Purkinje cells (By similarity). Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides.
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Database links
- Entrez Gene: 57120 Human
- Entrez Gene: 94221 Mouse
- Entrez Gene: 309774 Rat
- Omim: 606845 Human
- SwissProt: Q9HD26 Human
- SwissProt: Q3TAF1 Mouse
- SwissProt: Q8BH60 Mouse
- Unigene: 191539 Human
- Unigene: 390258 Mouse
see all
Target information above from: UniProt accession
Q9HD26
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CAL antibodyCFTR associated ligand antibodyCFTR-associated ligand antibody
- dJ94G16.2 antibodydJ94G16.2 PIST antibodyFIG antibodyFused in glioblastoma antibodyGolgi associated PDZ and coiled coil motif containing antibodyGolgi associated PDZ and coiled coil motif containing protein antibodyGolgi-associated PDZ and coiled-coil motif-containing protein antibodyGOPC 1 antibodyGOPC antibodyGOPC_HUMAN antibodyGOPC1 antibodyOTTHUMP00000040403 antibodyPDZ protein interacting specifically with TC 10 antibodyPDZ protein interacting specifically with TC10 antibodyPDZ/coiled coil domain binding partner for the rho family GTPase TC 10 antibodyPDZ/coiled coil domain binding partner for the rho family GTPase TC10 antibodyPIST antibodyProtein interacting specifically with Tc 10 antibodyProtein interacting specifically with Tc10 antibody
see all
Anti-PIST antibody images
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Western blot - PIST antibody (ab37036)Lane 1 : Anti-PIST antibody (ab37036) at 1 µg/ml
Lane 2 : Anti-PIST antibody (ab37036) at 2 µg/ml
Lane 3 : Anti-PIST antibody (ab37036) at 4 µg/ml
Lane 1 : rat colon cell lysate
Lane 2 : rat colon cell lysate
Lane 3 : rat colon cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 51 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PIST antibody (ab37036)ab37036 at 1µg/ml staining PIST in rat colon tissue by IHC
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Immunocytochemistry/ Immunofluorescence-Anti-PIST antibody(ab37036)Immunofluorescence of PIST in Rat Colon cells using ab37036 at 10 ug/ml. -
Immunocytochemistry/ Immunofluorescence-Anti-PIST antibody(ab37036)Immunofluorescence of PIST in Rat Colon cells using ab37036 at 10 ug/ml.
References for Anti-PIST antibody (ab37036)
This product has been referenced in:
- Xiao N et al. PICK1 deficiency causes male infertility in mice by disrupting acrosome formation. J Clin Invest 119:802-12 (2009). ICC/IF ; Rat, Mouse . Read more (PubMed: 19258705) »
