Overview

  • Product nameAnti-PIST antibodySee all PIST primary antibodies ...
  • Description
    Rabbit polyclonal to PIST
  • Specificityab37036 recognises PIST.
  • Tested applicationsWB, IHC-P, IHC-Fr, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide of 16 amino acid from near the carboxy terminus of PIST (Human)

  • Positive control
    • Rat colon cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab37036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IHC-P
IHC-Fr
ICC/IF
  • Application notesICC/IF: Use at an assay dependent dilution (PMID 19258705).
    IHC-P: Use at a concentration of 1 µg/ml.
    WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 51 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.
    • Tissue specificityUbiquitously expressed.
    • Involvement in diseaseNote=A chromosomal aberration involving GOPC is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.
    • Sequence similaritiesContains 1 PDZ (DHR) domain.
    • DomainThe PDZ domain mediates interactions with FZD5, FZD8, ACCN3, GRID2, CLCN3 (By similarity). Mediates also interaction with CFTR and ADRB1.
      The coiled-coil region probably mediates association to membranes, targeting to the Golgi, and interactions with GOLGA3, and STX6. May also mediate interaction with RHOQ.
    • Cellular localizationCytoplasm. Golgi apparatus membrane. Golgi apparatus > trans-Golgi network membrane. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Enriched in synaptosomal and postsynaptic densities (PSD) fractions (By similarity). Expressed in cell bodies and dendrites of Purkinje cells (By similarity). Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides.
    • Target information above from: UniProt accession Q9HD26 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • CAL antibody
      • CFTR associated ligand antibody
      • CFTR-associated ligand antibody
      • dJ94G16.2 antibody
      • dJ94G16.2 PIST antibody
      • FIG antibody
      • Fused in glioblastoma antibody
      • Golgi associated PDZ and coiled coil motif containing antibody
      • Golgi associated PDZ and coiled coil motif containing protein antibody
      • Golgi-associated PDZ and coiled-coil motif-containing protein antibody
      • GOPC 1 antibody
      • GOPC antibody
      • GOPC_HUMAN antibody
      • GOPC1 antibody
      • OTTHUMP00000040403 antibody
      • PDZ protein interacting specifically with TC 10 antibody
      • PDZ protein interacting specifically with TC10 antibody
      • PDZ/coiled coil domain binding partner for the rho family GTPase TC 10 antibody
      • PDZ/coiled coil domain binding partner for the rho family GTPase TC10 antibody
      • PIST antibody
      • Protein interacting specifically with Tc 10 antibody
      • Protein interacting specifically with Tc10 antibody
      see all

    Anti-PIST antibody images

    • Lane 1 : Anti-PIST antibody (ab37036) at 1 µg/ml
      Lane 2 : Anti-PIST antibody (ab37036) at 2 µg/ml
      Lane 3 : Anti-PIST antibody (ab37036) at 4 µg/ml

      Lane 1 : rat colon cell lysate
      Lane 2 : rat colon cell lysate
      Lane 3 : rat colon cell lysate

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 51 kDa
    • ab37036 at 1µg/ml staining PIST in rat colon tissue by IHC

    • Immunofluorescence of PIST in Rat Colon cells using ab37036 at 10 ug/ml.

    References for Anti-PIST antibody (ab37036)

    This product has been referenced in:
    • Xiao N  et al. PICK1 deficiency causes male infertility in mice by disrupting acrosome formation. J Clin Invest 119:802-12 (2009). ICC/IF ; Rat, Mouse . Read more (PubMed: 19258705) »

    See 1 Publication for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (testes)
    Specification testes
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.03% Triton X 100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    Verified customer

    Submitted Jan 07 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"