Overview

  • Product nameAnti-PIST antibodySee all PIST primary antibodies ...
  • Description
    Rabbit polyclonal to PIST
  • Specificityab37036 recognises PIST.
  • Tested applicationsWB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide of 16 amino acid from near the carboxy terminus of PIST (Human)

  • Positive control
    • Rat colon cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab37036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 51 kDa.
IHC-P Use a concentration of 1 µg/ml.
IHC-Fr 1/50.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionPlays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.
  • Tissue specificityUbiquitously expressed.
  • Involvement in diseaseNote=A chromosomal aberration involving GOPC is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.
  • Sequence similaritiesContains 1 PDZ (DHR) domain.
  • DomainThe PDZ domain mediates interactions with FZD5, FZD8, ACCN3, GRID2, CLCN3 (By similarity). Mediates also interaction with CFTR and ADRB1.
    The coiled-coil region probably mediates association to membranes, targeting to the Golgi, and interactions with GOLGA3, and STX6. May also mediate interaction with RHOQ.
  • Cellular localizationCytoplasm. Golgi apparatus membrane. Golgi apparatus > trans-Golgi network membrane. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Enriched in synaptosomal and postsynaptic densities (PSD) fractions (By similarity). Expressed in cell bodies and dendrites of Purkinje cells (By similarity). Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAL antibody
    • CFTR associated ligand antibody
    • CFTR-associated ligand antibody
    • dJ94G16.2 antibody
    • dJ94G16.2 PIST antibody
    • FIG antibody
    • Fused in glioblastoma antibody
    • Golgi associated PDZ and coiled coil motif containing antibody
    • Golgi associated PDZ and coiled coil motif containing protein antibody
    • Golgi-associated PDZ and coiled-coil motif-containing protein antibody
    • GOPC 1 antibody
    • GOPC antibody
    • GOPC_HUMAN antibody
    • GOPC1 antibody
    • OTTHUMP00000040403 antibody
    • PDZ protein interacting specifically with TC 10 antibody
    • PDZ protein interacting specifically with TC10 antibody
    • PDZ/coiled coil domain binding partner for the rho family GTPase TC 10 antibody
    • PDZ/coiled coil domain binding partner for the rho family GTPase TC10 antibody
    • PIST antibody
    • Protein interacting specifically with Tc 10 antibody
    • Protein interacting specifically with Tc10 antibody
    see all

Anti-PIST antibody images

  • Lane 1 : Anti-PIST antibody (ab37036) at 1 µg/ml
    Lane 2 : Anti-PIST antibody (ab37036) at 2 µg/ml
    Lane 3 : Anti-PIST antibody (ab37036) at 4 µg/ml

    Lane 1 : rat colon cell lysate
    Lane 2 : rat colon cell lysate
    Lane 3 : rat colon cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 51 kDa
  • ab37036 at 1µg/ml staining PIST in rat colon tissue by IHC

  • Immunofluorescence of PIST in Rat Colon cells using ab37036 at 10 ug/ml.

References for Anti-PIST antibody (ab37036)

This product has been referenced in:
  • Wang H  et al. Atg7 is required for acrosome biogenesis during spermatogenesis in mice. Cell Res 24:852-69 (2014). Mouse . Read more (PubMed: 24853953) »
  • Xiao N  et al. PICK1 deficiency causes male infertility in mice by disrupting acrosome formation. J Clin Invest 119:802-12 (2009). ICC/IF ; Rat, Mouse . Read more (PubMed: 19258705) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (testes)
Specification testes
Fixative Paraformaldehyde
Permeabilization Yes - 0.03% Triton X 100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jan 07 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"