The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionMay play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.
Involvement in diseaseDefects in PITX1 are a cause of congenital clubfoot (CCF) [MIM:119800]; also known as talipes equinovarus (TEV). Clubfoot is a congenital limb deformity defined as fixation of the foot in cavus, adductus, varus, and equinus (i.e. inclined inwards, axially rotated outwards, and pointing downwards) with concomitant soft tissue abnormalities. Clubfoot may occur in isolation or as part of a syndrome. Clubfoot appears to be a multifactorial trait.
Sequence similaritiesBelongs to the paired homeobox family. Bicoid subfamily. Contains 1 homeobox DNA-binding domain.