Overview

  • Product nameAnti-PITX1 antibodySee all PITX1 primary antibodies ...
  • Description
    Rabbit polyclonal to PITX1
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog
  • Immunogen

    A region within synthetic peptide: GTPASPYSVY RDTCNSSLAS LRLKSKQHSS FGYGALQGPA SGLNACQYNS, corresponding to C terminal amino acids 265-314 of Human PITX1

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab66943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMay play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.
  • Involvement in diseaseDefects in PITX1 are a cause of congenital clubfoot (CCF) [MIM:119800]; also known as talipes equinovarus (TEV). Clubfoot is a congenital limb deformity defined as fixation of the foot in cavus, adductus, varus, and equinus (i.e. inclined inwards, axially rotated outwards, and pointing downwards) with concomitant soft tissue abnormalities. Clubfoot may occur in isolation or as part of a syndrome. Clubfoot appears to be a multifactorial trait.
  • Sequence similaritiesBelongs to the paired homeobox family. Bicoid subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BFT antibody
    • CCF antibody
    • Hindlimb expressed homeobox protein backfoot antibody
    • Hindlimb-expressed homeobox protein backfoot antibody
    • Homeobox protein PITX1 antibody
    • LBNBG antibody
    • Paired like homeodomain 1 antibody
    • Paired like homeodomain transcription factor 1 antibody
    • Paired-like homeodomain transcription factor 1 antibody
    • Pituitary homeo box 1 antibody
    • Pituitary homeobox 1 antibody
    • Pituitary otx related factor antibody
    • Pitx1 antibody
    • PITX1_HUMAN antibody
    • POTX antibody
    • PTX1 antibody
    see all

Anti-PITX1 antibody images

  • All lanes : Anti-PITX1 antibody (ab66943) at 1.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-PITX1 antibody (ab66943)

ab66943 has not yet been referenced specifically in any publications.

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