Anti-PITX1/BFT antibody (ab70273)
Key features and details
- Rabbit polyclonal to PITX1/BFT
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PITX1/BFT antibody
See all PITX1/BFT primary antibodies -
Description
Rabbit polyclonal to PITX1/BFT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Chimpanzee, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human PITX1/BFT (N terminal).
Database link: NP_002644.3 -
Positive control
- HeLa whole cell lysate (ab150035)
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2500 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
|
|
IP |
Use at 1-4 µg/mg of lysate.
|
Notes |
---|
WB
1/2500 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa). |
IP
Use at 1-4 µg/mg of lysate. |
Target
-
Function
May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb. -
Involvement in disease
Defects in PITX1 are a cause of congenital clubfoot (CCF) [MIM:119800]; also known as talipes equinovarus (TEV). Clubfoot is a congenital limb deformity defined as fixation of the foot in cavus, adductus, varus, and equinus (i.e. inclined inwards, axially rotated outwards, and pointing downwards) with concomitant soft tissue abnormalities. Clubfoot may occur in isolation or as part of a syndrome. Clubfoot appears to be a multifactorial trait. -
Sequence similarities
Belongs to the paired homeobox family. Bicoid subfamily.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 5307 Human
- Omim: 602149 Human
- SwissProt: P78337 Human
- Unigene: 84136 Human
-
Alternative names
- BFT antibody
- CCF antibody
- Hindlimb expressed homeobox protein backfoot antibody
see all
Images
-
HeLa whole cell lysate (1 mg) was immunoprecipitated using ab70273 at 3 µg/mg lysate (lane 1) or an IgG control (lane 2). One quarter of the total IP reaction was loaded on to a gel and analysed by Western blot using ab70273 at 1 µg/ml. Bands were detected using chemiluminescence with an exposure time of 30 seconds.
-
All lanes : Anti-PITX1/BFT antibody (ab70273) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 34 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (9)
ab70273 has been referenced in 9 publications.
- Poos AM et al. PITX1 Is a Regulator of TERT Expression in Prostate Cancer with Prognostic Power. Cancers (Basel) 14:N/A (2022). PubMed: 35267575
- Ohira T et al. PITX1 inhibits the growth and proliferation of melanoma cells through regulation of SOX family genes. Sci Rep 11:18405 (2021). PubMed: 34526609
- Byun JS et al. The transcription factor PITX1 drives astrocyte differentiation by regulating the SOX9 gene. J Biol Chem 295:13677-13690 (2020). PubMed: 32759168
- Ohira T et al. PITX1 protein interacts with ZCCHC10 to regulate hTERT mRNA transcription. PLoS One 14:e0217605 (2019). PubMed: 31404068
- Lv X et al. Circular RNA circ-PITX1 promotes the progression of glioblastoma by acting as a competing endogenous RNA to regulate miR-379-5p/MAP3K2 axis. Eur J Pharmacol 863:172643 (2019). PubMed: 31493405
- Teng HW et al. Protein tyrosine phosphatase 1B targets PITX1/p120RasGAP thus showing therapeutic potential in colorectal carcinoma. Sci Rep 6:35308 (2016). PubMed: 27752061
- Pellicelli M et al. E2F1 and TFDP1 Regulate PITX1 Expression in Normal and Osteoarthritic Articular Chondrocytes. PLoS One 11:e0165951 (2016). WB ; Human . PubMed: 27802335
- Booy EP et al. The RNA helicase RHAU (DHX36) suppresses expression of the transcription factor PITX1. Nucleic Acids Res 42:3346-61 (2014). Human . PubMed: 24369427
- Picard C et al. Nuclear accumulation of prohibitin 1 in osteoarthritic chondrocytes down-regulates PITX1 expression. Arthritis Rheum 65:993-1003 (2013). PubMed: 23310948