Anti-PITX2 antibody (ab32832)

Overview

• Product nameAnti-PITX2 antibodySee all PITX2 primary antibodies ...
• Description
  Rabbit polyclonal to PITX2
• Tested applicationsELISA, WB, IHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cow, Dog
• Immunogen

  Synthetic peptide: SVLAPGQPRS LDSSKHRLEV HTISDTSSPE AAEKDKSQQG KNEDVGAEDP, corresponding to a region within N terminal amino acids 37-86 of Human PITX2

  Run BLAST with Run BLAST with
• Positive controlHepG2 cell lysate. Human intestine.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Developmental Biology
  • Embryogenesis
  • Axis formation and symmetry

  • Cardiovascular
  • Heart
  • Cardiogenesis
  • Transcription factors/regulators

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Developmental Families
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

Applications

Target

• FunctionMay play an important role in development and maintenance of anterior structures. Isoform PTX2C is involved in left-right asymmetry the developing embryo.
• Involvement in diseaseDefects in PITX2 are the cause of Axenfeld-Rieger syndrome type 1 (RIEG1) [MIM:180500]; also known as Rieger syndrome type 1. RIEG1 is an autosomal dominant defect characterized by hypodontia (partial anodontia), anal stenosis, hypertelorism, mental deficiency, agenesis of the facial bones, with malformation of the anterior chamber of the eye.
  Defects in PITX2 are the cause of iridogoniodysgenesis type 2 (IRID2) [MIM:137600]; also known as iridogoniodysgenesis syndrome 2 (IGDS2). It is an autosomal dominant inherited disease.
  Defects in PITX2 are a cause of Peters anomaly (PAN) [MIM:604229]. It is a congenital defect of the anterior chamber of the eye.
  Defects in PITX2 are associated with ring dermoid of cornea (RDC) [MIM:180550]. RDC is an autosomal dominantly inherited syndrome characterized by bilateral annular limbal dermoids with corneal and conjunctival extension.
• Sequence similaritiesBelongs to the paired homeobox family. Bicoid subfamily.
  Contains 1 homeobox DNA-binding domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q99697 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5308 Human
  • Entrez Gene: 5308 Human
  • Entrez Gene: 18741 Mouse
  • Entrez Gene: 54284 Rat
  • Omim: 601542 Human
  • SwissProt: Q3KQX9 Human
  • SwissProt: Q99697 Human
  • SwissProt: Q99697 Human

  • SwissProt: P97474 Mouse
  • SwissProt: Q9R0W1 Rat
  • Unigene: 643588 Human
  • Unigene: 246804 Mouse
  • Unigene: 17591 Rat

  see all

• Alternative names
  All1 responsive gene 1 antibodyALL1 responsive protein ARP1 antibodyALL1-responsive protein ARP1 antibody

  ARP 1 antibodyARP1 antibodyBrx 1 antibodyBrx1 antibodyHomeobox protein PITX2 antibodyIDG 2 antibodyIDG2 antibodyIGDS 2 antibodyIGDS antibodyIGDS2 antibodyIHG 2 antibodyIHG2 antibodyIRID 2 antibodyIRID2 antibodyMGC111022 antibodyMGC20144 antibodyOtlx 2 antibodyOtlx2 antibodyPaired like homeodomain transcription factor 2 antibodyPaired-like homeodomain transcription factor 2 antibodyPituitary homeo box 2 antibodyPituitary homeobox 2 antibodyPITX 2 antibodypitx2 antibodyPITX2_HUMAN antibodyPTX 2 antibodyPTX2 antibodyRGS antibodyRIEG 1 antibodyRIEG antibodyRieg bicoid related homeobox transcription factor 1 antibodyRIEG bicoid related homeobox transcription factor antibodyRIEG bicoid-related homeobox transcription factor antibodyRIEG1 antibodyRS antibodysolurshin antibody

  see all

Anti-PITX2 antibody images

• 

  Western blot - PITX2 antibody (ab32832)
  Anti-PITX2 antibody (ab32832) at 2.5 µg/ml + HepG2 cell lysate
  
  Secondary
  HRP conjugated anti Rabbit IgG at 1/50000 dilution
  
  Predicted band size : 35 kDa
  Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - PITX2 antibody (ab32832)
  ab32832 at a concentration of 4.0 µg/ml staining human PITX2 by Immunohistochemistry in paraffin embedded intestine tissue. Cells with positive label (as indicated by the arrows) are epithelial cells of the intestinal villus.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PITX2 antibody(ab32832)
  Immunohistochemistry of Human Intestine tissue at an antibody concentration of 4-8µg/ml using anti-PITX2 antibody (ab32832)