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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab21869? Please let us know so that we can cite the reference in this datasheet
ab21869 has been referenced in 1 publications.
- Yabuta Y et al. TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice. J Cell Biol 192:781-95 (2011). IHC-Fr; Mouse. PubMed: 21383078
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot
Lanes 1 - 2 : Anti-PIWIL4 antibody (ab21869) at 2 µg/ml
Lanes 3 - 4 : Anti-PIWIL4 antibody (ab21869) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027)
Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) with
Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027)
Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) with
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 98 kDa
Observed band size : 98 kDa
ab21869 specifically recognises PIWIL4 at 98kDa, which is demonstrated by the efficient blocking by the immunizing peptide
Immunocytochemistry/ Immunofluorescence - Anti-PIWIL4 antibody (ab21869)
ICC/IF image of ab21869 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab21869 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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