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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Overview
Product name
Anti-PIWIL4 antibody
See all PIWIL4 products (4) ...
Description
Rabbit polyclonal to PIWIL4
Tested applications
IHC-Fr, WBmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Mouse PIWIL4.
(Peptide available as ab23535.)
Positive control
Mouse testis lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution. (PubMed: 21383078)
WB: Use a concentration of 1 µg/mlDetects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).(Block with 5% BSA for 1 hour and incubate antibody in TBST for 1 hour or more. )
Target
Function
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci.
Tissue specificity
Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed.
Sequence similarities
Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain.
Post-translational
modifications
Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.
Cellular localization
Nucleus. Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization.
Target information above from: UniProt accessionQ7Z3Z4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp686P01248 antibody
- FLJ36156 antibody
- HILI 2 antibody
see all
Database links
- Entrez Gene: 143689 Human
- Entrez Gene: 330890 Mouse
- Entrez Gene: 689972 Rat
- Omim: 610315 Human
- SwissProt: Q7Z3Z4 Human
- SwissProt: Q8CGT6 Mouse
- SwissProt: Q4G033 Rat
- Unigene: 660188 Human
see all
Anti-PIWIL4 antibody images:
Western blot
Lanes 1 - 2 : Anti-PIWIL4 antibody (ab21869) at 2 µg/ml
Lanes 3 - 4 : Anti-PIWIL4 antibody (ab21869) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027)
Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) with PIWIL4 peptide (ab23535) at 1 µg/ml
Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027)
Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) with PIWIL4 peptide (ab23535) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 98 kDa
Observed band size : 98 kDa
ab21869 specifically recognises PIWIL4 at 98kDa, which is demonstrated by the efficient blocking by the immunizing peptide (ab23535).
References for Anti-PIWIL4 antibody (ab21869)
This product has been referenced in:
- Yabuta Yet al. TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice. J Cell Biol 192:781-95 (2011). IHC-Fr; Mouse.Read more (PubMed: 21383078) »
See 1 publication for this product
Publishing research using ab21869? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"