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Overview

  • Product namePIWIL4 peptide
  • Protein descriptionSynthetic peptide derived from within residues 800 to the C-terminus of Mouse PIWIL4.(Note: the amino acid sequence is proprietary)This peptide was used as an immunogen for ab21869 - PIWIL4 antibody.
  • Molecular weight95kDa
  • Protein length17 amino acids

    • Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferInformation available upon request.
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab23535 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Blocking
  • Application notesBL: Use at a concentration of 1 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        DKFZp686P01248FLJ36156HILI 2
        HILI2HIWI 2HIWI2Miwi 2 proteinMiwi2PIWIPiwi like 2Piwi like 4Piwi like 4 (Drosophila)PIWI like proteinPiwi like protein 4Piwi-like protein 4PIWILPIWIL 4PIWIL4PIWL4_HUMAN
      see all
  • FunctionPlays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci.
  • Tissue specificityExpressed in testis. According to PubMed:17544373, it is ubiquitously expressed.
  • Sequence similaritiesBelongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • Post-translational
    modifications    Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.
  • Cellular localizationNucleus. Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization.

    • Target information above from: UniProt accession Q7Z3Z4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    PIWIL4 peptide images

    • Western blot
      Western blot
      Lanes 1 - 2 : Anti-PIWIL4 antibody (ab21869) at 2 µg/ml
      Lanes 3 - 4 : Anti-PIWIL4 antibody (ab21869) at 1 µg/ml

      Lane 1 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027)
      Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) with PIWIL4 peptide (ab23535) at 1 µg/ml
      Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027)
      Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) with PIWIL4 peptide (ab23535) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) (ab28446) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 98 kDa
      Observed band size : 98 kDa

      See Abreview

    References for PIWIL4 peptide (ab23535)

    ab23535 has not yet been referenced specifically in any publications.

    Publishing research using ab23535 ? Please let us know so that we can cite the reference in this datasheet

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