![Western blot - PKA 2 beta (regulatory subunit) antibody [EP2649Y] (ab75993) image](#ab75993_WB_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKA 2 beta (regulatory subunit) antibody [EP2649Y] (ab75993) image](#ab75993_IHC_1.jpg)
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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Overview
Product name
Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y]
See all PKA 2 beta (regulatory subunit) products (8) ...
Description
Rabbit monoclonal [EP2649Y] to PKA 2 beta (regulatory subunit)
Tested applications
WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues near the C terminus of human PKA 2 beta (regulatory subunit).
Positive control
WB: Human brain lysate IHC-P: Human testis tissue
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP2649Y
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
Flow Cyt: 1/80.
ICC/IF: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of HRP conjugated or polymerized HRP secondary antibodies recommended. Stronger signals have been found using the polymerized HRP secondary.
IP: 1/60.
WB: 1/25,000 - 1/50,000. Predicted molecular weight: 46 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
PKA 2 beta (regulatory subunit or PRKAR2B) is one of the regulatory subunits of cAMP-dependent Protein Kinase (PKA), which is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription. PKA is composed of conserved catalytic subunits and regulatory subunits (R1 alpha, R1 beta, R2 alpha, and R2 beta). Upon adenylate cyclase activation, AMP binds to the regulatory subunits, and releases the catalytic subunit, which becomes active upon its dissociation from the regulatory subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.
Cellular localization
Cytoplasmic
Alternative names
- AI451071 antibody
- AW061005 antibody
- cAMP dependent protein kinase type II beta regulatory chain antibody
see all
Database links
- Entrez Gene: 5577 Human
- Entrez Gene: 19088 Mouse
- Entrez Gene: 24679 Rat
- Omim: 176912 Human
- SwissProt: P31323 Human
- SwissProt: Q6DHZ2 Human
- SwissProt: P31324 Mouse
- SwissProt: P12369 Rat
see all
Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y] images:
Western blot - PKA 2 beta (regulatory subunit) antibody [EP2649Y] (ab75993)
![Western blot - PKA 2 beta (regulatory subunit) antibody [EP2649Y] (ab75993)](/ps/datasheet/Images/75/ab75993/ab75993_WB_1.jpg)
Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y] (ab75993) at 1/50000 dilution + Human brain lysate at 10 µg
Secondary
goat anti rabbit HRP at 1/2000 dilution
Predicted band size : 46 kDa
Observed band size : 46 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKA 2 beta (regulatory subunit) antibody [EP2649Y] (ab75993)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKA 2 beta (regulatory subunit) antibody [EP2649Y] (ab75993)](/ps/datasheet/Images/75/ab75993/ab75993_IHC_1.jpg)
ab75993, at a 1/100 dilution, staining PKA 2 beta (regulatory subunit) in formalin fixed, paraffin embedded human testis tissue by Immunohistochemistry.
References for Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y] (ab75993)
This product has been referenced in:
- Nagalla Set al. Platelet microRNA-mRNA coexpression profiles correlate with platelet reactivity. Blood 117:5189-97 (2011). WB; Human.Read more (PubMed: 21415270) »
See 1 publication for this product
Publishing research using ab75993? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"