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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Overview
Product name
Anti-PKA 2 beta (regulatory subunit) antibody [RS30]
See all PKA 2 beta (regulatory subunit) products (8) ...
Description
Mouse monoclonal [RS30] to PKA 2 beta (regulatory subunit)
Tested applications
ELISA, IF, Indirect ELISAmore details
Cross reactivity
Reacts with
Cow
Immunogen
Purified regulatory subunit of cAMP-dependent protein kinase type II from bovine brain.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
RS30
Myeloma
P3x63-Ag8.653
Isotype
IgG2a
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
IF
Indirect ELISA
Application notes
ELISA: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
I-ELISA: Specific antibody titer is 1.5 x 106, using regulatory subunit from bovine source as a coating antigen, 2.5x105, using regulatory subunit from porcine source as a coating antigen and 2.0x103, using regulatory subunit from human source as a coating antigen. This clone does not inhibit catalytic activity. This clone activates cAMP-binding.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
PKA 2 beta (regulatory subunit or PRKAR2B) is one of the regulatory subunits of cAMP-dependent Protein Kinase (PKA), which is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription. PKA is composed of conserved catalytic subunits and regulatory subunits (R1 alpha, R1 beta, R2 alpha, and R2 beta). Upon adenylate cyclase activation, AMP binds to the regulatory subunits, and releases the catalytic subunit, which becomes active upon its dissociation from the regulatory subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.
Cellular localization
Cytoplasmic
Alternative names
- AI451071 antibody
- AW061005 antibody
- cAMP dependent protein kinase type II beta regulatory chain antibody
see all
Database links
- Entrez Gene: 282463 Cow
- SwissProt: P31322 Cow
- Unigene: 462 Cow
References for Anti-PKA 2 beta (regulatory subunit) antibody [RS30] (ab25862)
ab25862 has not yet been referenced specifically in any publications.
Publishing research using ab25862? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"