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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Overview
Product name
Anti-PKA 2 beta (regulatory subunit) antibody
See all PKA 2 beta (regulatory subunit) products (8) ...
Description
Rabbit polyclonal to PKA 2 beta (regulatory subunit)
Specificity
ab28256 recognizes Protein Kinase A Regulatory Subunit IIb. It does not recognize the other PKA regulatory subunits (PKARIa, PKARIIa, PKARIb).
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Rabbit, Human
Immunogen
Synthetic peptide (Rat) from PKA 2 beta (regulatory subunit). (Peptide available as ab41298.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-PKA 2 beta (regulatory subunit) antibody(ab28256)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab28256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
- 1 Image
IHC-P
Application notes
WB: 1/1000, when using colorometric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Predicted molecular weight: 46 kDa. But note that when used against the reduced protein, ab28256 identifies bands at 55 kDa and 53 kDa, as well as activation/breakdown products. Dilution optimised using Chromogenic detection.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
PKA 2 beta (regulatory subunit or PRKAR2B) is one of the regulatory subunits of cAMP-dependent Protein Kinase (PKA), which is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription. PKA is composed of conserved catalytic subunits and regulatory subunits (R1 alpha, R1 beta, R2 alpha, and R2 beta). Upon adenylate cyclase activation, AMP binds to the regulatory subunits, and releases the catalytic subunit, which becomes active upon its dissociation from the regulatory subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.
Cellular localization
Cytoplasmic
Alternative names
- AI451071 antibody
- AW061005 antibody
- cAMP dependent protein kinase type II beta regulatory chain antibody
see all
Database links
- Entrez Gene: 5577 Human
- Entrez Gene: 19088 Mouse
- Entrez Gene: 24679 Rat
- Omim: 176912 Human
- SwissProt: P31323 Human
- SwissProt: Q6DHZ2 Human
- SwissProt: P31324 Mouse
- SwissProt: P12369 Rat
see all
Anti-PKA 2 beta (regulatory subunit) antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-PKA 2 beta (regulatory subunit) antibody(ab28256)
Ab28256 staining human normal breast tissue. Staining is localized to cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
References for Anti-PKA 2 beta (regulatory subunit) antibody (ab28256)
ab28256 has not yet been referenced specifically in any publications.
Publishing research using ab28256? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"